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首页> 外文期刊>Plant and Soil >Determination of nitrogen fixation effectiveness in selected Medicago truncatula isolates by measuring nitrogen isotope incorporation into pheophytin
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Determination of nitrogen fixation effectiveness in selected Medicago truncatula isolates by measuring nitrogen isotope incorporation into pheophytin

机译：通过测量氮同位素掺入脱镁叶绿素中的截短苜蓿分离株的固氮效率

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摘要

Effectiveness is a term used to describe the input that a bacterial nitrogen-fixing symbiosis makes to plant nitrogen metabolism. In legumes, effectiveness is considered a polymorphic trait where specific interactions between the plant and symbiotic rhizobia contribute to the success of the interaction. Evaluation of effectiveness using model legumes like Medicago truncatula may open new avenues for genetic studies. In previous work, an isotope dilution mass spectrometry method, which uses the effect of nitrogen fixation on the nitrogen isotope composition of chlorophyll in plants grown on 15N fertilizer as a measure of effectiveness, was developed for estimating the contribution of symbiotic nitrogen fixation to plant nitrogen content. This 15N-dilution assay was used to evaluate the level of nitrogen fixation effectiveness in three Medicago truncatula lines that have been used as parents in generating recombinant inbred lines. Three Sinorhizobium meliloti strains, USDA 1600, 102F51 and MK506, differ in this measure of effectiveness on three lines of M. truncatula: Jemalong A17, DZA315.16 and F83005.5. Plant–rhizobia combinations grown in two different conditions showed comparable differences in effectiveness.
机译：有效性是一个术语，用于描述细菌固氮共生对植物氮代谢的影响。在豆科植物中，有效性被认为是一种多态性状，其中植物与共生根瘤菌之间的特定相互作用有助于相互作用的成功。使用截叶苜蓿等模型豆类进行有效性评估可能会为基因研究开辟新的途径。在先前的工作中，开发了一种同位素稀释质谱法，该方法利用固氮作用对15 N肥料下种植的植物中叶绿素的氮同位素组成的影响，以此来评估共生作用。固氮到植物的氮含量。使用这种15 N稀释法评估了三株run藜苜蓿品系中的固氮效果水平，这些品系被用作亲本，产生了重组自交系。在三种截枝分枝M上，Jemalong A17，DZA315.16和F83005.5的三种苜蓿中华根瘤菌菌株USDA 1600、102F51和MK506在有效性方面有所不同。在两种不同条件下生长的植物-根瘤菌组合显示出可比的功效差异。

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