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首页> 外文期刊>Plant and Soil >Increases in phosphoenolpyruvate carboxylase activity in iron-deficient sugar beet roots: Analysis of spatial localization and post-translational modification
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Increases in phosphoenolpyruvate carboxylase activity in iron-deficient sugar beet roots: Analysis of spatial localization and post-translational modification

机译：缺铁甜菜根中磷酸烯醇丙酮酸羧化酶活性的增加：空间定位和翻译后修饰的分析

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摘要

Root tips of Fe-deficient and Fe-sufficient sugar beet plants grown in hydroponics have been used to study the changes in the amount and activity of the cytosolic enzyme phosphoenolpyruvate carboxylase (PEPC, EC 4.1.1.31). Phosphoenolpyruvate carboxylase activity in extracts of the yellow Fe-deficient root tips was, at pH 7.3, 30-fold higher (when expressed on a FW basis) and 7.1-fold higher (when expressed on a protein basis) than that found in the extracts of Fe-sufficient root tips. The amount of phosphoenolpyruvate carboxylase protein determined by immuno-blotting was, on a protein basis, 35-fold larger in the yellow zone of Fe-deficient root tips than in the Fe-sufficient root tips. The inhibition of the phosphoenolpyruvate carboxylase activity by 500 μm malate was 41 and 58% in the extracts Fe-deficient and Fe-sufficient roots. The possibility that post-translational regulation of phosphoenolpyruvate carboxylase may occur mediated through phosphorylation, was studied by immunological detection of phosphoserine residues in root tip extracts.
机译：在水培法中生长的缺铁和缺铁的甜菜植株的根尖已被用于研究胞溶酶磷酸烯醇丙酮酸羧化酶（PEPC，EC 4.1.1.31）的数量和活性的变化。黄色铁缺乏根尖的提取物中的磷酸烯醇丙酮酸羧化酶活性在pH 7.3下比在提取物中发现的高30倍（以FW表示时）和7.1倍（以蛋白质表示时）。铁充足的根尖。以蛋白质为基础，通过免疫印迹测定的磷酸烯醇丙酮酸羧化酶蛋白的量是铁不足的根尖的黄色区域中铁含量充足的根尖的35倍。铁缺乏和铁充足的根中，500μm苹果酸对磷酸烯醇丙酮酸羧化酶活性的抑制作用分别为41％和58％。通过免疫学检测根尖提取物中磷酸丝氨酸残基，研究了磷酸烯醇丙酮酸羧化酶的翻译后调控可能通过磷酸化介导的可能性。

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