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首页> 外文期刊>Plasma processes and polymers >States of Biological Components in Bacteria and Bacteriophages during Inactivation by Atmospheric Dielectric Barrier Discharges
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States of Biological Components in Bacteria and Bacteriophages during Inactivation by Atmospheric Dielectric Barrier Discharges

机译：大气介电势垒放电灭活过程中细菌和噬菌体中生物成分的状态

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摘要

Atmospheric DBD has been applied to the wet state of Escherichia coli and bacteriophage-λ. Upon DBD treatment, both E.coli and λphage were immediately inactivated. The states of different biological components were monitored during the course of inactivation. Only minor and slow degradation of proteins, DNA, and membranes was observed, a remarkable degradation was seen only after the completion of sterilization. Analysis of GFP recombinantly introduced into E.coli cells proved that the DBD has a prominent protein denaturation activity without affecting peptide bonds. The irreversible denaturation of proteins, seen in the early stage of DBD application, may play a central role in inactivation of both the bacteria and bacteriophages.
机译：大气DBD已应用于大肠杆菌和噬菌体λ的潮湿状态。经DBD处理后，大肠杆菌和λ噬菌体均立即失活。在灭活过程中监测不同生物成分的状态。仅观察到蛋白质，DNA和膜的轻微和缓慢降解，仅在灭菌完成后才观察到显着降解。重组导入大肠杆菌细胞的GFP的分析证明，DBD具有显着的蛋白质变性活性，且不影响肽键。在DBD应用的早期阶段，蛋白质的不可逆变性可能在细菌和噬菌体的失活中起重要作用。

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