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Hydrolytic enzyme-producing microbes in the Antarctic oligochaete Grania sp (Annelida)

机译:南极寡食Grania sp(Annelida)中产生水解酶的微生物

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The oligochaete Grania sp. is a common inhabitant of Artigas Beach at Maxwell Bay (King George Island, South Shetland Islands, Maritime Antarctica) that proliferate during the summer season and feed on debris of red and brown algae. This investigation was undertaken to test the hypothesis that Grania sp. has an enzyme-producing microbiota that may facilitate the worm's nutrient uptake by processing or metabolizing macroalgae compounds. A culture-based approach was used to investigate the occurrence of microorganisms able to degrade proteins, lipids and polysaccharides. Thirty-four hydrolytic enzyme-producing microorganisms associated with these worms, including bacteria and yeasts, were isolated and identified by sequencing a partial fragment of the 16S and 26S rDNA genes, respectively. These microorganisms have the ability to produce extracellular proteases, esterases, amylases, cellulases and agarases. The microbial genera found during this work (Flavobacterium, Pseudomonas, Salinibacterium, Psychrobacter, Cystobasidium and Rhodotorula) have been previously described in association with red and brown Antarctic algae. Our results suggest that this microbiome has a digestive capability that may assist Grania sp. in metabolizing nutrients from algae, leading us to consider the possibility of a mutualistic relationship between them. The association between the worm Grania sp. and a cold-active hydrolyzing microbiota may contribute to the macroalgae decomposition and nutrient recycling in the Antarctic ecosystem.
机译:oligochaete Grania sp.。是位于麦克斯韦湾(乔治王岛,南设得兰群岛,南极海事)的阿蒂加斯海滩的常见居民,在夏季扩散,并以红色和棕色藻类的碎片为食。进行这项研究以检验Grania sp。拥有一种产生酶的微生物群,可通过加工或代谢大型藻类化合物来促进蠕虫的养分吸收。基于培养的方法用于研究能够降解蛋白质,脂质和多糖的微生物的发生。通过分别对16S和26S rDNA基因的部分片段进行测序,分离并鉴定了与这些蠕虫相关的34种产生水解酶的微生物,包括细菌和酵母。这些微生物具有产生细胞外蛋白酶,酯酶,淀粉酶,纤维素酶和琼脂酶的能力。先前已经描述了在这项工作中发现的微生物属(黄细菌,假单胞菌,盐杆菌,精神杆菌,蓝藻和红斑杜鹃)与红色和棕色南极藻有关。我们的结果表明,该微生物组具有消化功能,可能有助于Grania sp.。代谢藻类中的营养成分,这使我们考虑了它们之间相互关系的可能性。蠕虫Grania sp。之间的关联。冷活性水解菌群可能有助于南极生态系统中的大型藻类分解和养分循环。

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