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首页> 外文期刊>Polar biology >Erythrocyte nuclear abnormalities and leukocyte profile in the Antarctic fish Notothenia coriiceps after exposure to short- and long-term heat stress
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Erythrocyte nuclear abnormalities and leukocyte profile in the Antarctic fish Notothenia coriiceps after exposure to short- and long-term heat stress

机译：长期和短期热应激后南极鱼类Notothenia coriiceps的红细胞核异常和白细胞特征

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Several studies suggest that Antarctic endemic stenothermic fish species, such as Notothenia coriiceps, have a limited ability to acclimate to warmer conditions. In the present study, we evaluated the effects of exposure to short- and long-term temperature increases on the occurrence of erythrocytic nuclear abnormalities (ENAs) and changes in the leukocyte profile of N. coriiceps. For the experiments, 30 specimens of N. coriiceps were captured in Admiralty Bay and transferred to the Commandant Ferraz Brazilian Antarctic Station where they were acclimated to water at 0 A degrees C for 6 days and subsequently subjected to treatments of temperature increases (2-4 A degrees C) on a short- (1 day) or long- (6 days) term basis. At the end of exposure, peripheral blood samples were collected and blood smears were prepared for qualitative and quantitative analysis of the ENAs and the leukocyte profile. Significant responses were observed for both ENAs and leukocyte profile. The responses of ENAs were characterized by an increased incidence of bilobed nuclei in erythroblasts following 1-day exposure to water at 2-4 A degrees C, as well as increased incidence of bilobed nuclei in erythrocytes following 1-day and 6-day exposure to water at 4 A degrees C. In the leukocyte profile, 1-day exposure to water at 2 A degrees C promoted a stress response characterized by diminished levels of circulating lymphocytes (lymphopenia) and elevated levels of circulating monocytes (monocytosis). In conclusion, our results indicate that elevation of water temperature during short periods provoked cellular and physiological stress in N. coriiceps. Furthermore, we conclude that in terms of nuclear alterations, erythrocytes and erythroblasts respond differently to thermal stress. Finally, we conclude that the methodology proposed was efficient in quantifying thermal stress in N. coriiceps and we recommend the utilization of this technique in the biomonitoring of thermal stress in this species and other notothenioids.
机译：几项研究表明，南极地方性狭窄鱼类，例如Notothenia coriiceps，适应气候变暖的能力有限。在本研究中，我们评估了短期和长期体温升高对红皮细胞核异常（ENA）的发生和N. coriiceps白细胞谱变化的影响。为了进行实验，在金钟湾捕获了30头N. coriiceps的标本，并将其转移到指挥官Ferraz巴西南极站，在那里将它们在0 A摄氏度的水中适应了6天，然后进行了温度升高的处理（2-4短期（1天）或长期（6天）的摄氏度。在暴露结束时，收集外周血样品并准备血液涂片以对ENA和白细胞谱进行定性和定量分析。对于ENA和白细胞谱均观察到显着反应。 ENA的响应的特征是，在2-4 A摄氏度的水中暴露1天后，成红细胞中双叶核的发生率增加，并且在暴露1天和6天后，红细胞中双叶核的发生率增加。在4 A摄氏度的水中。在白细胞特征中，在2 A摄氏度的水中暴露1天会促进应激反应，其特征在于循环淋巴细胞的水平降低（淋巴细胞减少）和循环单核细胞水平升高（单核细胞增多）。总之，我们的结果表明，短期内水温的升高会引起N. coriicepseps的细胞和生理压力。此外，我们得出结论，就核改变而言，红细胞和成红细胞对热应激的反应不同。最后，我们得出的结论是，所提出的方法可有效量化N. coriiceps的热应激，并且我们建议将该技术用于该物种和其他类异戊二烯对热应激的生物监测。

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