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首页> 外文期刊>Polar biology >Marine archaeal community structure from Potter Cove, Antarctica: high temporal and spatial dominance of the phylum Thaumarchaeota
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Marine archaeal community structure from Potter Cove, Antarctica: high temporal and spatial dominance of the phylum Thaumarchaeota

机译：来自南极洲波特湖的海洋古细菌群落结构：Thaumarchaeota门的高度时空优势

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摘要

Archaeal communities represent a significant fraction of the Antarctic marine microbial plankton and surely play a relevant role in the proper functioning of the ecosystem. We studied the archaeal community structure in surface water samples from Potter Cove, Antarctica. Temporal and spatial variability was investigated along a whole year cycle using DGGE and 16S rRNA gene sequencing from clone libraries. Additionally, photosyn-thetic pigments, suspended particulate matter (SPM), salinity and temperature were measured. The multivariate analysis performed using diversity, dominance and richness indexes, and environmental data evidenced a seasonal pattern in the archaeal community and revealed that spring-summer samples clustered separately from autumn to winter ones. High salinity and high values of diversity and richness were related to autumn-winter samples, whereas the spring-summer samples were associated mainly with higher values of temperature, SPM, Chl-a, carotenoids and archaeal dominance. The phylogenetic analysis of five independent clone libraries (467 sequences) showed that 448 sequences fell into a clade containing Nitrosopumilus maritimus and other sequences of ammonia-oxidizing archaea which belong to the Thaumarchaeota phylum. A high fraction of these sequences (62 %) constituted a single cluster containing only highly similar Potter Cove representatives, which probably belong to the same species. Fifteen sequences were affiliated to a group closely related to the order Thermoplasmatales (Eur-yarchaeota). This work represents a first step towards obtaining a deep understanding of the structure of archaeal communities from Antarctic coastal marine environments and contributes to cover the current gap in knowledge of the dynamics of the archaeoplankton in the Antarctic seas.
机译：古细菌群落代表了南极海洋微生物浮游生物的很大一部分，并且肯定在生态系统的正常运转中起着重要的作用。我们研究了南极洲波特湖地表水样品中的古细菌群落结构。使用克隆库中的DGGE和16S rRNA基因测序，研究了整个一年的时间和空间变异性。此外，还测量了光合色素，悬浮颗粒物（SPM），盐度和温度。使用多样性，优势度和丰富度指数以及环境数据进行的多变量分析证明了古细菌群落的季节性模式，并揭示了春夏样品从秋季到冬季分别聚类。高盐度，高多样性和丰富度值与秋冬样品有关，而春夏季样品主要与温度，SPM，Chl-a，类胡萝卜素和古细菌优势值较高有关。对五个独立克隆文库（467个序列）的系统发育分析表明，有448个序列落入包含Nitrosopumilus maritimus以及其他属于Thaumarchaeota phylum的氨氧化古细菌序列的进化枝。这些序列中的很大一部分（62％）构成一个仅包含高度相似的Potter Cove代表的集群，这些代表可能属于同一物种。 15个序列隶属于与热质亚目（Eur-yarchaeota）密切相关的一组。这项工作代表了从南极沿海海洋环境中深入了解古细菌群落结构的第一步，并有助于弥补目前在南极海中浮游生物动力学知识方面的空白。

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  • 来源
    《Polar biology》 |2015年第2期|117-130|共14页
  • 作者

    Edgardo A. Hernandez; Anouk M.-T. Piquet; Jose L. Lopez; Anita G. J. Buma; Walter P. Mac Cormack;

  • 作者单位

    Departamento de Microbiologia Ambiental, Instituto Antartico Argentino, Balcarce 290, C1064AAF Ciudad Autonoma de Buenos Aires, Argentina,Facultad de Farmacia y Bioquimica, Catedra de Biotecnologia e Instituto de Nanobiotecnologia UBA-CONICET, Universidad de Buenos Aires, Junin 956 piso 6, C1113AAD Ciudad Autonoma de Buenos Aires, Argentina;

    Department of Ocean Ecosystems, Energy and Sustainability Research Institute, University of Groningen, Groningen, The Netherlands;

    Catedra de Virologia, Facultad de Farmacia y Bioquimica, Universidad de Buenos Aires, Junin 956 piso, C1113AAD Ciudad Autonoma de Buenos Aires, Argentina;

    Department of Ocean Ecosystems, Energy and Sustainability Research Institute, University of Groningen, Groningen, The Netherlands;

    Departamento de Microbiologia Ambiental, Instituto Antartico Argentino, Balcarce 290, C1064AAF Ciudad Autonoma de Buenos Aires, Argentina,Facultad de Farmacia y Bioquimica, Catedra de Biotecnologia e Instituto de Nanobiotecnologia UBA-CONICET, Universidad de Buenos Aires, Junin 956 piso 6, C1113AAD Ciudad Autonoma de Buenos Aires, Argentina;

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  • 正文语种 eng
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  • 关键词

    Antarctic Peninsula; 16S rDNA; DGGE; Marine archaea;

    机译：南极半岛;16S rDNA;DGGE;海洋古细菌;

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