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首页> 外文期刊>Polar biology >A molecular survey of freshwater microeukaryotes in an Arctic reservoir (Svalbard, 79°N) in summer by using next-generation sequencing
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A molecular survey of freshwater microeukaryotes in an Arctic reservoir (Svalbard, 79°N) in summer by using next-generation sequencing

机译：夏季利用下一代测序技术对北极水库（斯瓦尔巴特群岛，北纬79°）中的淡水微真核生物进行分子调查

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摘要

The molecular diversity of freshwater microeukaryotes, particularly phytoplankton, in the Arctic Svalbard, has been relatively unexplored. Freshwater algae are considered biological indicators of environmental change and can be useful in assessing the impact of global climate change and increased environmental pollution. In this study, freshwater microeukaryotes in an Arctic reservoir at Ny-Alesund (Svalbard, Norway) were studied using the hypervariable V1-V3 small subunit rRNA and 454 py-rosequencing. On the basis of 8,956 reads, we revealed high genetic diversity in eukaryotes, representing all known eukaryotic supergroups, except Excavata. "Chromalveola-ta" (previously supergroup Chromalveolata) and Archaep-lastida were the most and least abundant supergroups, respectively. After data mining, 57 phylotypes were detected from 7,398 pyrosequences. They were dominated by stramenopiles (84 %)and Dinofiagellata(13 %), with minor contributions from Cryptophyta, Chlorophyta, and Telone-mida. The detection of algae belonging to the orders Mamiellales and Monomastigales provides a window into a fraction of the 'rare biosphere' that had previously been undetected in such environments. Interestingly, no hapto-phytes were recorded. Stramenopiles and Dinofiagellata mainly comprised taxa belonging to the families Chryso-phyceae, Synurophyceae, and Dinophyceae. On the basis of the proportion of operational taxonomic units, the dominant phylotypes were found to include Ochromonas spp., Mal-lomonas spp., and Uroglena americana. These results demonstrate the significance of a chrysophyte-dominated microeukaryotic community, which is of great potential for future studies in terms of reconstruction of past climate trends and monitoring of environmental change in the Arctic.
机译：北极斯瓦尔巴特群岛的淡水微真核生物，尤其是浮游植物的分子多样性尚未得到充分研究。淡水藻类被认为是环境变化的生物学指标，可用于评估全球气候变化和加剧的环境污染的影响。在这项研究中，使用高变V1-V3小亚基rRNA和454 py-rosequencing对位于Ny-Alesund（挪威斯瓦尔巴特群岛）的北极水库中的淡水微真核生物进行了研究。根据8,956篇文献，我们揭示了真核生物中的高遗传多样性，代表了除Excavata外的所有已知的真核超群。 “ Chromalveola-ta”（以前是Chromalveolata的超群）和Archaep-lastida分别是最丰富和最不丰富的超群。数据挖掘后，从7398个焦磷酸序列中检测到57个系统型。它们以稻草虫（84％）和Dinofiagellata（13％）为主，其中隐孢子虫，绿藻和端粒螨的贡献较小。对属于Mamiellales和Monomastigales目的藻类的检测提供了进入以前在此类环境中未发现的“稀有生物圈”的一部分的窗口。有趣的是，没有记录到触藻。伞形目和Dinofiagellata主要包括类群，属于金藻科，滑藻科和恐龙科。根据操作分类单元的比例，主要的系统型包括Ochromonas spp。，Mal-lomonas spp。和Uroglena americana。这些结果证明了以金藻为主导的微真核生物群落的意义，就重建过去的气候趋势和监测北极的环境变化而言，这在未来的研究中具有巨大的潜力。

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