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首页> 外文期刊>Polar biology >Molecular characterization of the chalcone isomerase gene family in Deschampsia antarctica
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Molecular characterization of the chalcone isomerase gene family in Deschampsia antarctica

机译：南极Deschampsia中查尔酮异构酶基因家族的分子表征

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摘要

Deschampsia antarctica (Poaceae) is one of the two vascular plants known to have colonized the Antarctic region. Studies examining the biosynthesis of flavonoids, compounds which plants use, for example, for protection against overexposure to UV light or as antioxidants that scavenge free radicals and other oxidative species, in D. antarctica may provide clues to its success in that extreme environment. We characterized the family of genes encoding chalcone isomerase (CHI EC 5.5.1.6), an important enzyme involved in flavonoid biosynthesis, in D. antarctica. Sequence analysis of the three family members revealed differences in numbers of introns and lengths of coding regions among the three and suggest that DaCHI3 is likely a pseudogene (ΨDaChi2). Salinity stress resulted in differential mRNA expression of the DaCHI genes with ΨDaCHI2 exhibiting the earliest response (3-h post-treatment), induced by as much as sevenfold, while DaCHI1 and DaCHI2 mRNAs accumulated later (3d and 5d post-treatment, respectively) and, in the case of DaCHI2, with a response of nearly sixfold. We discuss how differences in the proposed gene structures, deduced protein characteristics, and mRNA expression patterns suggest that the members of this gene family may have unique functions in the phenylpropanoid pathway in D. antarctica.
机译：南极Deschampsia（Poaceae）是已知在南极地区定居的两种维管植物之一。研究植物类黄酮的生物合成的研究，例如植物用于防止紫外线过度暴露或用作南极衣藻的清除自由基和其他氧化性物质的抗氧化剂，可能为在这种极端环境下取得成功提供了线索。我们表征了南极衣藻中编码查尔酮异构酶（CHI EC 5.5.1.6）的基因家族，查尔酮异构酶是参与类黄酮生物合成的重要酶。对三个家族成员的序列分析揭示了三个家族中内含子数量和编码区长度的差异，并表明DaCHI3可能是假基因（ΨDaChi2）。盐胁迫导致DaCHI基因差异表达，其中ΨDaCHI2表现出最早的反应（治疗后3小时），诱导多达7倍，而DaCHI1和DaCHI2 mRNA的积累较晚（分别为治疗后3d和5d）。而对于DaCHI2，响应将近六倍。我们讨论如何在拟议的基因结构，推断的蛋白质特征和mRNA表达模式的差异表明该基因家族的成员可能在南极D.的苯丙烷途径中具有独特的功能。

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  • 来源
    《Polar biology》 |2013年第9期|1269-1280|共12页
  • 作者

    Pablo Zamora; Ariel Pardo; Angelica Fierro; Humberto Prieto; Gustavo E. Zuniga;

  • 作者单位

    Plant Sciences Department, University of California, Davis, CA, USA ,Universidad de Tarapaca, Arica, Chile;

    Departamento de Biologia, Universidad de Santiago de Chile, Santiago, Chile;

    Departamento de Quimica Organica, Facultad de Quimica, Pontificia Universidad Catolica de Chile, Santiago, Chile;

    La Platina Research Center, Instituto de Investigaciones Agropecuarias, Santiago, Chile;

    Departamento de Biologia, Universidad de Santiago de Chile, Santiago, Chile;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Chalcone isomerase; Phenylpropanoids; Deschampsia antarctica; Gene family; Pseudogene;

    机译：查尔酮异构酶;苯丙烷;南极Deschampsia;基因家族;假基因;

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