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Phylogenetic diversity of sediment bacteria in the northern Bering Sea

机译：白令海北部沉积物细菌的系统发育多样性

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摘要

The bacterial diversity in sediments from the northern Bering Sea was investigated by culture-independent approaches. Community fingerprint analysis by polymerase chain reaction-denaturing gradient gel electro-phoresis (PCR-DGGE) revealed that sediment at two deep stations (DBSE and DBS1, >400 m in depth) harbored a bacterial community distinct from the sediments collected at shallow stations (<150 m in depth) on the continental shelf. Three 16S rRNA gene clone libraries for sediments collected from shallow to deep water stations (NEC5, DBSE and DBS1, respectively) were established. Sediment collected at the deepest station DBS1 showed the highest diversity index value. Sequences fell into 19 major lineages of the domain Bacteria: Alpha-, Beta-, Gamma-, Delta- and Epsilonproteobacteria, Bacteroidetes, Acidobacteria, Actinobacteria, Firmicutes, Planctomycetes, Nitrospirae, Verrucomicrobia, Chloroflexi, Chlorobi, Spirochaetes, Cyanobacteria (or chloroplasts), and candidate divisions OP8, TM6, and WS3. A small fraction of retrieved sequences (1.8%) did not fall into any taxonomic division. Deltaproteobacteria (30%) was the dominant phylum in the three libraries, followed by Gammaproteobacteria (21%) and Acidobacteria (16%). The percentages of cloned sequences with the highest similarity to reported sequences below 97 and 93% were 48.1 and 24.3%, respectively. A large number of phylotypes affiliated with bacteria that play important roles in the carbon, sulfur, and nitrogen cycles suggest an important link of bacteria to the matter cycling in these subarctic sediments.
机译：通过与文化无关的方法研究了白令海北部沉积物中的细菌多样性。通过聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）进行的群落指纹分析表明，两个深处（DBSE和DBS1，深度> 400 m）的沉积物具有不同于浅处收集的沉积物的细菌群落（深度<150 m）。建立了三个16S rRNA基因克隆文库，用于从浅水站到深水站（分别为NEC5，DBSE和DBS1）收集的沉积物。在最深站DBS1收集的沉积物显示出最高的多样性指数值。序列分为细菌领域的19个主要谱系：α-，β-，γ-，δ和Epsilon变形细菌，拟杆菌，酸杆菌，放线菌，硬毛，扁菌，硝化螺旋藻，疣状微生物，绿变藻，绿弯曲菌，螺旋藻，蓝藻，以及候选部门OP8，TM6和WS3。检索到的序列的一小部分（1.8％）未归类于任何分类。在这三个文库中，Deltaproteobacteria（30％）是占主导地位的门，其次是Gammaproteobacteria（21％）和Acidobacteria（16％）。与报告的序列相似性最高的克隆序列（低于97％和93％）的百分比分别为48.1和24.3％。与细菌有关的大量系统型在碳，硫和氮循环中起重要作用，这表明细菌与这些弧下沉积物中物质循环的重要联系。

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  • 来源
    《Polar biology》 |2011年第6期|p.907-919|共13页
  • 作者

    Yinxin Zeng; Yang Zou; Bo Chen; Jacqueline M. Grebmeier; Huirong Li; Yong Yu; Tianling Zheng;

  • 作者单位

    Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136 Shanghai, China,Key Laboratory of MOE for Coast and Wetland Ecosystem,School of Life Sciences, Xiamen University,361005 Xiamen, China;

    Key Laboratory of MOE for Coast and Wetland Ecosystem,School of Life Sciences, Xiamen University,361005 Xiamen, China;

    Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136 Shanghai, China;

    Chesapeake Biological Laboratory,University of Maryland Center for Environmental Science,Solomons, MD 20688, USA;

    Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136 Shanghai, China;

    Key Laboratory for Polar Science of State Oceanic Administration, Polar Research Institute of China, 200136 Shanghai, China;

    Key Laboratory of MOE for Coast and Wetland Ecosystem,School of Life Sciences, Xiamen University,361005 Xiamen, China;

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  • 正文语种 eng
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  • 关键词

    Bacterial diversity; PCR-DGGE; 16S rRNA gene clone library; Sediment; Northern Bering Sea;

    机译：细菌多样性;PCR-DGGE;16S rRNA基因克隆文库沉淀;白令海北部;

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