Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR

Tae-Gyu Park; Elanor M. Bell; Imojen Pearce; Parke A. Rublee - Christopher J. S. Bolch; Gustaaf M. Hallegraeff

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Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR

机译：实时荧光定量PCR检测南极盐湖中一种新的生态型菲菲（Dinphyceae）

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The heterotrophic dinoflagellate Pfiesteria piscicida was detected in Ace Lake in the Vestfold Hills, eastern Antarctica by using real-time PCR based on 18S rDNA sequences. Antarctic water samples collected in 2004 were tested by species-specific real-time PCR assays for the identification of P. piscicida and P. shumwayae. Positive results were shown with P. piscicida-spedfic real-time PCR, and PCR products were examined by sequence analysis for confirmation. A phylogenetic tree made from partial 18S rDNA sequences showed that the Antarctic clone clustered with P. piscicida. This result suggests that P. piscicida is present in the extreme conditions of an Antarctic saline lake which has not contained fish for thousands of years.

机译：使用基于18S rDNA序列的实时PCR技术，在南极东部Vestfold Hills的Ace湖中检测到异养的鞭毛藻Pfiesteria piscicida。 2004年收集的南极水样本通过物种特异性实时PCR分析进行了检测，以鉴定P. piscicida和P. shumwayae。疫霉实时定量PCR显示阳性结果，并通过序列分析检查PCR产物以确认。用部分18S rDNA序列构建的系统发育树表明，南极克隆与P. piscicida聚在一起。该结果表明，P。piscicida存在于南极盐湖的极端条件下，该湖数千年来一直没有鱼类。

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《Polar biology》 |2007年第7期|p.843-848|共6页
作者
Tae-Gyu Park; Elanor M. Bell; Imojen Pearce; Parke A. Rublee - Christopher J. S. Bolch; Gustaaf M. Hallegraeff;
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School of Plant Science, University of Tasmania, Private Bag 55, Hobart, TAS 7001, Australia;

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正文语种 eng
中图分类植物的地区分布;动物的地区分布;
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1. Detection of Heterotrophic Dinoflagellate Pfiesteria piscicida (Dinophyceae) in Surface Water Samples Using Real-time PCR [J] . Tae-Gyu Park, Yang-Soon Kang, Mi-Kyung Seo, Journal of Fisheries Science and Technology . 2008,第4期

机译：使用实时荧光定量PCR检测地表水样品中的异养鞭毛藻（Dinflagellate piscicida（Dinophyceae））
2. Identification of Pfiesteria piscicida (Dinophyceae) and Pfiesteria-like organisms using internal transcribed spacer-specific PCR assays [J] . Litaker RW., Vandersea MW., Kibler SR., Journal of phycology . 2003,第4期

机译：使用内部转录间隔子特异性PCR分析法鉴定小叶菲氏菌（Dinophyceae）和类菲氏菌
3. Development of Real-Time PCR Assays for Rapid Detection of Pfiesteria piscicida and Related Dinoflagellates [J] . Holly A. Bowers, Torstein Tengs, Howard B. Glasgow, Applied and Environmental Microbiology . 2000,第11期

机译：实时PCR检测法快速检测费氏小孢子虫和相关鞭毛藻的开发
4. Research of different saline-alkaline soils on methanotrophs special activity by real-time QPCR [C] . Mingde Yang, Yan Jiao, Xin Li, International Conference on Energy Equipment Science and Engineering . 2015

机译：实时QPCR对不同盐碱土壤不同盐碱土壤的研究
5. Detection and characterization of harmful algae by bioluminescent stress fingerprinting (Karlodinium micrum, Pfiesteria piscicida, Chattonella marina, Prorocentrum minimum, Akashiwo sanguinea). [D] . Wang, Jing. 2004

机译：通过生物发光应力指纹图谱（Karlodinium micrum，Pfiesteria piscicida，Chantellaella marina，Prorocentrum minimum，Akashiwo sanguinea）检测和表征有害藻类。
6. Development of Real-Time PCR Assays for Rapid Detection of Pfiesteria piscicida and Related Dinoflagellates [O] . Holly A. Bowers, Torstein Tengs, Howard B. Glasgow Jr., 2002

机译：实时PCR检测法快速检测费氏小孢子虫和相关鞭毛藻的开发
7. Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR [O] . Park, TG, Bell, EM, Pearce, I, 2007

机译：实时荧光定量PCR检测南极盐湖中一种新的生态型菲菲（Dinphyceae）

Detection of a novel ecotype of Pfiesteria piscicida (Dinophyceae) in an Antarctic saline lake by real-time PCR

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