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首页> 外文期刊>Polar biology >Fungal diversity in maritime Antarctic soils determined using a combination of culture isolation, molecular fingerprinting and cloning techniques
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Fungal diversity in maritime Antarctic soils determined using a combination of culture isolation, molecular fingerprinting and cloning techniques

机译：结合培养分离，分子指纹图谱和克隆技术确定的海洋南极土壤真菌多样性

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摘要

The diversity and phylogenetic relationships of fungi obtained from Antarctic soils were analysed using molecular techniques. Direct extraction of soil community DNA from two locations, Fossil Bluff (FB) and Jane Col (JC), was supplemented with isolation studies. Nucleic acids from both the community DNA and the colony extracts were PCR amplified using primers specific for the 18S rRNA gene (18S rDNA). Amplicons were separated in denaturant gels (DGGE) or following endonuclease digestion (ARDRA). Clones presenting unique ARDRA banding patterns and unique DGGE bands were sequenced. Comparison of the experimental sequences from the different techniques employed with those held online resulted in the repeated recovery of a limited range of related organisms indicating low species diversity of microfungi in these soils. A total of 102 fungal sequences were obtained from FB (37 sequences) and JC (65 sequences) that together were distributed among the Basidiomy-cota (48 sequences), Ascomycota (48 sequences) and Zygomycota (6 sequences). Sequences of the latter were only recovered from the JC soils. Phylogenetic comparisons of the experimental sequences with those held online have shown high rRNA gene relatedness with those obtained from other, less extreme, environments.
机译：使用分子技术分析了从南极土壤中获得的真菌的多样性和系统发育关系。从两个地点Fossil Bluff（FB）和Jane Col（JC）的直接提取土壤群落DNA补充了分离研究。使用对18S rRNA基因具有特异性的引物（18S rDNA）对来自社区DNA和菌落提取物的核酸进行PCR扩增。在变性凝胶（DGGE）中或在核酸内切酶消化后（ARDRA）分离扩增子。对呈现出独特的ARDRA带状模式和独特的DGGE带的克隆进行了测序。将来自不同技术的实验序列与在线获得的序列进行比较，结果表明有限范围的相关生物得以重复回收，表明这些土壤中微真菌的物种多样性较低。从FB（37个序列）和JC（65个序列）获得了总共102个真菌序列，它们一起分布在担子菌（48个序列），子囊（48个序列）和合子（6个序列）之间。后者的序列仅从JC土壤中回收。与在线保存的实验序列的系统进化比较表明，rRNA基因与从其他不太极端的环境获得的rRNA基因高度相关。

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