...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Polar biology >Degradation of nonane by bacteria from Antarctic marine sediment
【24h】

Degradation of nonane by bacteria from Antarctic marine sediment

机译：南极海洋沉积物中细菌对壬烷的降解

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A microbial enrichment culture tolerant of petroleum hydrocarbons was developed from sediment collected near Casey Station, Antarctica. To select cold-adapted microbes that would degrade diesel, enrichments were cultured at 0degreesC during six successive transfers to fresh medium, with Special Antarctic Blend diesel (SAB) as the sole carbon source. Biodegradation of components of the SAB was then measured in microcosms inoculated with the enrichment culture. After 16 weeks, the amount of biodegradation was small, but nonane (a C9 alkane) had degraded significantly more in inoculated microcosms than in sterile controls. DNA was then extracted from the enrichment cultures and a fragment of the 16S rRNA gene was amplified for denaturing gradient gel electrophoresis. Bands were excised from the gel and, following sequencing, were found to belong to the genera Pseudomonas and Colwellia.
机译：从南极州凯西站附近收集的沉积物中开发了一种耐石油烃的微生物富集培养物。为了选择能使柴油降解的冷适应微生物，在特殊的南极混合柴油（SAB）作为唯一碳源的连续六次转移到新鲜培养基的过程中，将浓缩物在0℃下进行培养。然后在接种富集培养物的微观世界中测量SAB组分的生物降解。 16周后，生物降解量很小，但是壬烷（一种C9烷烃）在接种的微观世界中的降解比在无菌对照组中的降解要大得多。然后从富集培养物中提取DNA，并扩增16S rRNA基因的片段，以使梯度凝胶电泳变性。从凝胶上切下条带，并在测序后发现其属于假单胞菌属和Colwellia属。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号