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首页> 外文期刊>Polar biology >A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica P|12
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A comparative transcriptomic analysis provides insights into the cold-adaptation mechanisms of a psychrophilic yeast, Glaciozyma antarctica P|12

机译：比较转录组学分析提供了对嗜冷酵母南极Glaciozyma P | 12的冷适应机制的见解。

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摘要

Glaciozyma antarctica PI12, a psychrophilic yeast from Antarctica, grows well at low temperatures. However, it is not clear how it responds and adapts to cold and freeze stresses. Hence, this project was set out to determine thecold-adaptation strategies and mechanisms of G. antarctica PI12 using a transcriptomic analysis approach. G. antarctica PI12 cells, grown in rich medium at 12 degrees C, were exposed to freeze stress at 0 and - 12 degrees C for 6 h and 24 h. Their transcriptomes were sequenced and analyzed. A hundred and sixty-eight genes were differentially expressed. The yeast gene expression patterns were found to be dependent on the severity of the cold with more genes being differentially expressed at - 12 degrees C than at 0 degrees C. Glaciozyma antarctica PI12 was found to share some common adaptation strategies with other yeasts, Saccharomyces cerevisiae and Mrakia spp., but at the same time, found to have some of its own unique strategies and mechanisms. Among the unique mechanisms was the production of antifreeze protein to prevent ice-crystallization inside and outside the cell. In addition, several molecular chaperones, detoxifiers of reactive oxygen species (ROS), and transcription and translation genes were constitutively expressed in G. antarctica PI12 to enable the cells to endure the fluctuating freezing temperatures. Interestingly, G. antarctica PI12 used nitrite as an alternative terminal acceptor of electrons when the oxygen level was low to minimize disruption of energy production in the cell. These mechanisms coupled with several other common mechanisms ensured that G. antarctica PI12 adapted well to the cold temperatures.
机译：南极洲的嗜冷酵母Glaciozyma antarctica PI12在低温下生长良好。但是，尚不清楚其如何响应和适应寒冷和冰冻的压力。因此，该项目的目的是采用转录组分析方法确定南极G. PI12的冷适应策略和机制。在12°C的丰富培养基中生长的南极G.PI12细胞在0和-12°C下暴露于冰冻应力下6小时和24小时。他们的转录组被测序和分析。一百六十八个基因被差异表达。发现酵母基因表达模式取决于感冒的严重程度，更多的基因在-12°C时比在0°C时差异表达。发现南极Glazyzyma PI12与其他酵母（Saccharomyces cerevisiae）有一些共同的适应策略。和Mrakia spp。，但同时发现它们有一些独特的策略和机制。独特的机制包括产生抗冻蛋白，以防止细胞内部和外部的冰晶。另外，在南极洲PI12中组成性表达了几种分子伴侣，活性氧（ROS）的解毒剂以及转录和翻译基因，以使细胞能够承受不断变化的冷冻温度。有趣的是，当氧气水平低时，南极洲种PI12将亚硝酸盐用作电子的替代末端受体，以最大程度地减少电池中能量产生的中断。这些机制与其他几种常见机制相结合，确保了南极毕赤酵母PI12很好地适应了寒冷的温度。

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