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首页> 外文期刊>Acta Societatis Botanicorum Poloniae >BIOCHEMICAL ACTIVITY OF AUXINS IN DEPENDENCE OF THEIR STRUCTURES IN WOLFFIA ARRHIZA (L.) WIMM
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BIOCHEMICAL ACTIVITY OF AUXINS IN DEPENDENCE OF THEIR STRUCTURES IN WOLFFIA ARRHIZA (L.) WIMM

机译:Wolffia Arrhiza(L.)WIMM中辅助蛋白的生物化学活性与其结构的关系

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摘要

Wolffia arrhiza (L.) Wimm. (Lemnaceae) as a mixotrophic plant reacts considerably weaker to used auxins with different chemical structures than typical photosynthetic vascular plants and algae especially from Chloro-phyta. Among used auxin compounds, the highest stimulative activity on W. arrhiza growth and biochemical parameters which were analysed in biomass, can be attributed to phenylacetic acid (PAA), a somewhat smaller to indole-3-acetic acid (IAA) and the smallest to 2-naphthaleneacetic acid (NAA) used in optimal concentration of 10~(-6) M, in comparison with the control culture, devoid of exogenous auxins. The investigated auxins, especially PAA and IAA, were found to have the most powerful stimulative activity (prevailingly between the 10th and the 15th day of cultivation) on the content of reducing sugars between 127 and 169%, chlorophyll a and b from 117 to 125%, total carotenoids from 115 to 132% and net photosynthetic rate from 127 to 144% in comparison with the control culture, which was treated as 100% for reference. However, the content of water-soluble proteins as well as nucleic acids (DNA and RNA) in the biomass of W. arrhiza was less effectively stimulated, hardly from 110 to 116% when compared to the control culture (100%).
机译:Wolffia arrhiza(L.) (Lemnaceae)作为一种混养植物,与典型的光合维管植物和藻类(尤其是绿藻类)相比,对具有不同化学结构的用过的生长素的反应相当弱。在用过的生长素化合物中,生物量中分析出的对小叶白屈菜生长和生化参数的最高刺激活性可归因于苯乙酸(PAA),苯丁酸(IAA)稍小,而吲哚-3-乙酸(IAA)最小。与对照培养相比,2-萘乙酸(NAA)的最佳浓度为10〜(-6)M,没有外源生长素。发现所研究的植物生长素,尤其是PAA和IAA,对还原糖含量在127%至169%之间,叶绿素a和b从117%至125%具有最强的刺激活性(主要在栽培的第10天至第15天之间)。与对照培养物相比,总类胡萝卜素的百分含量为115%至115%至132%,净光合速率为127%至144%,以100%作为参考。但是,小叶黄杨生物量中水溶性蛋白质以及核酸(DNA和RNA)的含量受到的刺激较差,与对照培养物(100%)相比几乎没有从110%增至116%。

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