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首页> 外文期刊>Polish Journal of Environmental Studies >Enzymatic Profile Aactivity of Grass Carp (Ctenopharyngodon Idella) After Exposure to the Pollutant Named Atrazine (Herbicide)
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Enzymatic Profile Aactivity of Grass Carp (Ctenopharyngodon Idella) After Exposure to the Pollutant Named Atrazine (Herbicide)

机译：暴露于名为阿特拉津（除草剂）的污染物后，草鱼（Ctenopharyngodon Idella）的酶学活性

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摘要

The present study contemplates the enzymatic profile of grass carp, including lactate dehydrogenase (LDH), creatinine phosphokinases (CPK), serum glutamic-pyruvic transaminase (SGPT), and alkaline phosphatase (Alk Phosp) under atrazine's acute toxicity effects (LC50) for 01 (15 mu l/L), 02 (13 mu l/L), 03 (10 mu l/L), and 04 (08 mu l/L) days/concentration, respectively. For analyzing the enzymatic profile we followed the biochemical analyzer set protocol (Merck micro lab 300 biochemistry analyzer) in the laboratory. Control group concentrations for LDH, CPK, SGPT, and Alk Phosp were 342 IU/ml, 7513.3 IU/ml, 46 mmol/l, and 126.6 IU/ml, respectively. After treatment LDH concentrations were 906, 851, 765, and 545 IU/ml, respectively. CPK concentrations were 1,737, 2,445, 3,718, and 5,767 IU/ml, respectively. SGPT concentrations were 27, 24.3, 13.67, and 8.67, respectively, and Alk Phosp concentrations were 50.3, 30, 22.3, and 17.6 IU/ml, respectively. Maximum inclined (P <= 0.001) in concentration of LDH was observed after 24 hrs exposure because of hepatic tissue damage, resulting in increased membrane permeability causing enhanced leaching out of LDH and as LDH participates in an anaerobic pathway, so increase LDH mean increases of anaerobic metabolism resulting from depletion of energy under environmental stress conditions by atrazine, while other enzymatic components like CPK, SGPT, and Alk Phosp showed kindred attributes in their result, like all parameter concentrations showed perpetual decline (P <=.0.001) in their concentrations indicating reduced enzymatic activity due to a reduction in permeability for these enzymes, forcing the enzymes to accumulate in the cells as well as decrease in enzyme synthesis due to intoxication of atrazine.
机译：本研究预期草鱼的酶学特征包括阿特拉津在01的急性毒性作用（LC50）下的乳酸脱氢酶（LDH），肌酐磷酸激酶（CPK），血清谷氨酸-丙酮酸转氨酶（SGPT）和碱性磷酸酶（Alk Phosp）。 （15μl/ L），02（13μl/ L），03（10μl/ L）和04（08μl/ L）天/浓度。为了分析酶促分布图，我们在实验室中遵循了生化分析仪设置规程（Merck micro lab 300生化分析仪）。对照组的LDH，CPK，SGPT和Alk Phosp浓度分别为342 IU / ml，7513.3 IU / ml，46 mmol / l和126.6 IU / ml。处理后，LDH浓度分别为906、851、765和545 IU / ml。 CPK浓度分别为1,737、2,445、3,718和5,767 IU / ml。 SGPT浓度分别为27、24.3、13.67和8.67，Alk Phosp浓度分别为50.3、30、22.3和17.6 IU / ml。暴露24小时后，由于肝组织损伤，导致LDH浓度最大倾斜（P <= 0.001），这导致膜通透性增加，导致LDH淋溶增强，并且LDH参与厌氧途径，因此LDH平均值增加阿特拉津在环境压力条件下消耗能量导致的厌氧代谢，而其他酶促成分（如CPK，SGPT和Alk Phosp）在其结果中显示出相似的属性，如所有参数浓度都显示其浓度永久下降（P <=。0.001）这表明由于这些酶的渗透性降低而降低了酶活性，由于阿特拉津的中毒，迫使这些酶在细胞中积累以及酶的合成减少。

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