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首页> 外文期刊>Polymer Degradation and Stability >Unusual degradation behavior of citric acid-crosslinked gelatin in vitro and in vivo
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Unusual degradation behavior of citric acid-crosslinked gelatin in vitro and in vivo

机译：柠檬酸交联明胶在体外和体内的异常降解行为

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摘要

The aim of this study was to evaluate the biodegradability and biocompatibility of gelatin matrices designed for drug-eluting stents (DESs). The matrices were prepared by crosslinking alkali-treated gelatin (AlGelatin) with a citric acid-based crosslinker, trisuccinimidyl citrate (TSC), to form AlGelatin-TSC. The biodegradation behavior of the matrices was evaluated in vitro and in vivo. An in vitro enzymatic degradation test showed that AlGelatin-TSC prepared at a TSC concentration of 20 mM is the most stable in collagenase solution compared to AlGelatin-TSC prepared at TSC concentrations higher or lower than 20 mM. Then, AlGelatin-TSC were implanted subcutaneously in rats to evaluate their biodegradability and tissue reaction in vivo. Similar to the in vitro degradation behavior, AlGelatin-TSC with TSC concentration of 20 mM exhibited the lowest biodegradable rate in vivo among all AlGelatin-TSC. In addition, strong inflammation and calcification were not observed for AlGelatin-TSC at any TSC concentration. From an analysis of the crosslinking density of the resulting AlGelatin-TSC, the lowest biodegradability of AlGelatin-TSC with TSC concentration of 20 mM was due to the highest crosslinking density of the matrix. These results suggest that AlGelatin-TSC is suitable for use as matrices in DESs because of its excellent biocompatibility and biodegradability.
机译：这项研究的目的是评估专为药物洗脱支架（DES）设计的明胶基质的生物降解性和生物相容性。通过将碱处理的明胶（AlGelatin）与柠檬酸基交联剂柠檬酸三琥珀酰亚胺酯（TSC）交联以形成AlGelatin-TSC，制备基质。在体外和体内评估基质的生物降解行为。体外酶促降解试验表明，与以高于或低于20 mM的TSC浓度制备的AlGelatin-TSC相比，以20 mM的TSC浓度制备的AlGelatin-TSC在胶原酶溶液中最稳定。然后，将AlGelatin-TSC皮下植入大鼠体内，以评估其生物降解性和体内组织反应。与体外降解行为相似，TSC浓度为20 mM的AlGelatin-TSC在所有AlGelatin-TSC中表现出最低的体内生物降解率。另外，在任何TSC浓度下，AlGelatin-TSC均未观察到强烈的炎症和钙化。从所得的AlGelatin-TSC的交联密度的分析中，TSC浓度为20mM的AlGelatin-TSC的生物降解性最低是由于基质的最高交联密度。这些结果表明，AlGelatin-TSC具有出色的生物相容性和生物降解性，因此适合用作DES中的基质。

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  • 来源
    《Polymer Degradation and Stability》 |2010年第10期|p.2088-2092|共5页
  • 作者

    Motoki Inoue; rnMakoto Sasaki; rnTetsushi Taguchi;

  • 作者单位

    Biomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan;

    rnBiomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan Graduate School of Pure and Applied Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan;

    rnBiomaterials Center, National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan Graduate School of Pure and Applied Science, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8577, Japan International Center for Materials Nanoarchitectonics (MANA), National Institute for Materials Science, 1-1 Namiki, Tsukuba, Ibaraki 305-0044, Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    gelatin; matrices; citric acid; biodegradation; crosslinking density;

    机译：明胶;矩阵柠檬酸;生物降解交联密度;

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