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Unusual change in molecular weight of polyhydroxyalkanoate (PHA) during cultivation of PHA-accumulating Escherichia coli

机译:积累PHA的大肠杆菌培养过程中聚羟基链烷酸酯(PHA)分子量的异常变化

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摘要

This study aimed to investigate the factors affecting molecular weight of poly[(R)-3-hydroxybutyrate] [P(3HB)1 when polyhydroxyalkanoate (PHA) synthase (PhaRCasp) from Bacillus sp. INT005 was used for P(3HB) synthesis in Escherichia coli JM109. It was found that the molecular weight of P(3HB) decreased with time in mid- and late-phase of culture and was strongly affected by culture temperature. At 37 ℃ culture temperature, the molecular weight of P(3HB) rapidly decreased from 4.4 × 105 to 4.8 × 104 with culture time, whereas it was almost unchanged at 25 ℃. Kinetic analysis suggested that the decrease in molecular weight of P(3HB) was due to random scission of the polymer chain. The decrease in molecular weight of P(3HB) was not observed when PHA synthases other than PhaRCesp were expressed. This study sheds light on the unique behaviour in molecular weight change of P(3HB) that is synthesized by E. coli expressing PhaRCasp.
机译:本研究旨在调查芽孢杆菌中的聚羟基链烷酸酯(PHA)合酶(PhaRCasp)时影响聚[(R)-3-羟基丁酸酯] [P(3HB)1]分子量的因素。 INT005用于大肠杆菌JM109中的P(3HB)合成。结果发现,P(3HB)的分子量在培养的中后期随着时间的推移而降低,并且受培养温度的强烈影响。培养温度为37℃时,P(3HB)的分子量随培养时间迅速从4.4×105降至4.8×104,而在25℃时几乎没有变化。动力学分析表明,P(3HB)分子量的降低是由于聚合物链的随机断裂造成的。当表达除PhaRCesp以外的PHA合成酶时,未观察到P(3HB)分子量的降低。这项研究揭示了表达PhaRCasp的大肠杆菌合成的P(3HB)分子量变化的独特行为。

著录项

  • 来源
    《Polymer Degradation and Stability》 |2010年第12期|p.2250-2254|共5页
  • 作者单位

    Department of Innovative and Engineered Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan;

    Asian Center for Environmental Research, Meisei University, 2-1-1 Hodokubo, Hino-shi, Tokyo 191-8506, Japan;

    Department of Innovative and Engineered Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan;

    Department of Innovative and Engineered Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan;

    Bioplastic Research Team, RIKEN Biomass Engineering Program, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan;

    Department of Innovative and Engineered Materials, Tokyo Institute of Technology, 4259 Nagatsuta, Midori-ku, Yokohama 226-8502, Japan;

    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan;

    Division of Biotechnology and Macromolecular Chemistry, Graduate School of Engineering, Hokkaido University, N13W8, Kita-ku, Sapporo 060-8628, Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    polyhydroxyalkanoate; PHA synthase; bacillus; molecular weight; random scission;

    机译:聚羟基链烷酸酯PHA合酶;芽孢杆菌分子量随机断言;

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