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首页> 外文期刊>Proceedings of the institution of mechanical engineers >Manipulation with atomic force microscopy: DNA and yeast micro/ nanoparticles in biological environments
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Manipulation with atomic force microscopy: DNA and yeast micro/ nanoparticles in biological environments

机译：原子力显微镜操作：生物环境中的DNA和酵母微/纳米颗粒

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摘要

Although significant attempts have been made to automate the manipulation of biological cells with atomic force microscopy, these efforts have faced many limitations and restrictions. Researchers have recently tried to measure the interacting forces in order to improve the reliability of manipulation operations with atomic force microscopy. By manipulation of microanoparticles of different materials and shapes, as the target particles, in different biological environments, the interacting forces existing between these microanoparticles and the biological environment will be different from those in the air medium. Therefore, in this paper, first, a general overview and simulation of the important forces acting in the biological environment was presented, and forces such as the adhesion force, hydration force and the electrostatic double-layer force were modeled and simulated in different environments (e.g. water, alcohol and blood plasma). After different biological environments (in comparison with the air medium) were explored and modeled, the manipulation operations with atomic force microscopy were simulated for different microanoparticles such as gold, DNA and yeast by considering the forces existing in various biological environments. The results of manipulation in this paper indicate that in the air and liquid environments, the biological particles (DNA and yeast) will start to move after a longer time and by a higher magnitude force relative to the gold particles. This outcome is predictable, given the properties and stickiness of the biological particles. Also by comparing the obtained results, it is found that the critical force and critical time of manipulation with atomic force microscopy for gold nanoparticles slightly increase in water relative to air, which can be due to the properties of water and the existing forces in water that resist against the movement of nanoparticles. Finally, the obtained results were compared with the available empirical results. It can be seen that the obtained simulation values have a good correlation with the empirical results. The closeness of these two values confirms the validity of the performed simulation.
机译：尽管人们已经进行了大量尝试以原子力显微镜自动操作生物细胞，但是这些努力面临许多限制和限制。研究人员最近尝试测量相互作用力，以提高原子力显微镜操作操作的可靠性。通过在不同的生物环境中操纵不同材料和形状的微米/纳米颗粒作为目标颗粒，这些微米/纳米颗粒与生物环境之间存在的相互作用力将不同于空气介质中的相互作用力。因此，本文首先对生物环境中的重要作用力进行了概述和仿真，并在不同环境中对诸如粘附力，水合力和静电双层力等作用力进行了建模和仿真（例如水，酒精和血浆）。在探索和建模了不同的生物环境（与空气介质相比）之后，考虑了各种生物环境中存在的力，对原子，显微技术对不同的微/纳米粒子（例如金，DNA和酵母）进行了模拟操作。本文的处理结果表明，在空气和液体环境中，相对于金颗粒，生物颗粒（DNA和酵母菌）将在更长的时间和更大的力作用下开始移动。考虑到生物颗粒的性质和粘性，这种结果是可预测的。此外，通过比较获得的结果，发现原子力显微镜对金纳米颗粒的临界力和临界操作时间相对于空气在水中略有增加，这可能是由于水的性质以及水中存在的力所致。抵抗纳米粒子的运动。最后，将获得的结果与可用的经验结果进行比较。可以看出，获得的仿真值与经验结果具有良好的相关性。这两个值的接近程度确认了所执行模拟的有效性。

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