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首页> 外文期刊>Proceedings of the institution of mechanical engineers >Separation and detection of urinary proteins by two-dimensional electrophoresis on the microfluidic chip
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Separation and detection of urinary proteins by two-dimensional electrophoresis on the microfluidic chip

机译：微流控芯片上二维电泳分离和检测尿蛋白

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摘要

A novel separating and detecting method of urinary proteins by non-gel sieving electrophoresis-free flow electrophoresis on the microfluidic chip was proposed in this article. Human serum albumin and human transferrin labeled by fluor-escein isothiocyanate were mixed and taken as the synthesized urinary protein samples. Factors affected on separation, such as the rate of electric fields applied in two dimension, the addition in the buffer, the concentration, and pH of buffer, were optimized, that is, 10% (w/v) dextran, 0.5% ethanediamine, 0.2 mol L~(-1) Tris-boric acid, pH 9.3, the ratio of the applied electric field in two dimensions 1:4.8, respectively. Fluorescein isothiocyanate-human serum albumin and fluores-cein isothiocyanate-transferrin were efficiently separated in 2 min, and the resolution of two proteins achieved was 2.2 by the designed microfluidic chip. Then, the urine samples of nephropathy patient were analyzed by the proposed non-gel sieving electrophoresis-free flow electrophoresis on the microfluidic chip. The results matched well with those detected by common clinical methods.
机译：本文提出了一种在微流控芯片上通过无凝胶筛分的无电泳电泳技术分离和检测尿蛋白的新方法。混合用异硫氰酸荧光素标记的人血清白蛋白和人转铁蛋白，作为合成的尿蛋白样品。优化了影响分离的因素，例如二维施加的电场速率，在缓冲液中的添加量，缓冲液的浓度和pH值，即10％（w / v）的右旋糖酐，0.5％的乙二胺， 0.2 mol L〜（-1）三硼酸，pH 9.3，二维电场的比例分别为1：4.8。异硫氰酸荧光素-人血清白蛋白和异硫氰酸荧光素-转铁蛋白在2分钟内有效分离，所设计的微流控芯片实现了两种蛋白质的分离度为2.2。然后，通过在微流控芯片上建议的无凝胶筛分的无电泳电泳技术分析肾病患者的尿液样品。结果与通过常规临床方法检测到的结果非常吻合。

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  • 作者

    Yanan Ni; Jun Gan; Yi Xu;

  • 作者单位

    Department of Pharmacy, Chemistry and Chemical Engineering College, Chongqing University, Chongqing, China,Department of Pharmacy, Defense Key Disciplines Lab of Novel Micronano Devices and System Technology, Chongqing University, Chongqing, China;

    Department of Pharmacy, Chemistry and Chemical Engineering College, Chongqing University, Chongqing, China,Department of Pharmacy, Defense Key Disciplines Lab of Novel Micronano Devices and System Technology, Chongqing University, Chongqing, China;

    Department of Pharmacy, Chemistry and Chemical Engineering College, Chongqing University, Chongqing 400030, China,Department of Pharmacy, Chemistry and Chemical Engineering College, Chongqing University, Chongqing, China;

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  • 原文格式 PDF
  • 正文语种 eng
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  • 关键词

    Non-gel sieving electrophoresis; free flow electrophoresis; microfluidic chip; urinary proteins;

    机译：非凝胶筛分电泳;自由流电泳微流控芯片尿蛋白;

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