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首页> 外文期刊>Proceedings of the institution of mechanical engineers >Impedance characterization and modelling of an improved patch clamp device
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Impedance characterization and modelling of an improved patch clamp device

机译:改进的膜片钳装置的阻抗表征和建模

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摘要

The gold standard for studying the properties of ion channels is the patch clamp, a technique that measures pA currents passing through ion channels in an isolated patch of cell membrane. The authors sought to design an improved device for whole-cell patch clamp recordings using nanoscale pipettes which more readily form seals with the cell membrane and a movable metal electrode to decrease the access resistance, increase the signal bandwidth, and clear debris from the pipette tip. Electrical characterization of the instrument by impedance spectroscopy showed that advancing the metal electrode towards the pipette tip decreased the impedance distinctly in different frequency bands, the greatest decrease occurring between 1 and 100 kHz, and the smallest at > 100 kHz. Finite element simulations of the electrode, incorporating the electrode/electrolyte interface, suggest that optimal signal transmission can be achieved with a thick-walled pipette, nearly filled with a large Ag/AgCl electrode, whose impedance can be reduced through surface modification.
机译:研究离子通道特性的金标准是膜片钳,该技术可测量通过隔离的细胞膜片中离子通道的pA电流。作者试图设计一种改进的设备,用于使用纳米级移液器进行全细胞膜片钳记录,该移液器更容易与细胞膜和可移动金属电极形成密封,以降低进入阻力,增加信号带宽并清除移液器尖端的碎屑。 。通过阻抗谱对仪器进行电学表征表明,将金属电极移向移液器吸头可明显降低不同频段的阻抗,最大的降低发生在1至100 kHz之间,最小的降低是> 100 kHz。电极的有限元模拟,结合了电极/电解质界面,表明使用厚壁移液器几乎可以装满一个大的Ag / AgCl电极,可以实现最佳的信号传输,通过表面改性可以降低其阻抗。

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