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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Brain-derived neurotrophic factor promotes the survival of neurons arising from the adult rat forebrain subependymal zone.
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Brain-derived neurotrophic factor promotes the survival of neurons arising from the adult rat forebrain subependymal zone.

机译：脑源性神经营养因子促进成年大鼠前脑室管膜下区产生的神经元的存活。

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摘要

Neuronal precursor cells persist in the adult forebrain ependymal/subependymal zone (SZ) and have been found to produce neurons in cultures derived from birds, rodents, and humans. We postulated that the survival of neurons generated from these cells might be constrained in adulthood by the local absence of trophic support. To test this hypothesis, we established explant cultures of adult rat forebrain SZ and assessed the effect of defined neurotrophins on the survival of new neurons arising from these explants. We found that microtubule-associated protein 2+ neurons arose from explants derived from a wide area of the SZ, spanning the rostral 6 mm of the ventricular system. In cultures exposed to brain-derived neurotrophic factor (BDNF), > 35% of new neurons survived at 22 days in vitro (DIV), and > 25% survived at 42 DIV, concurrent with the virtually complete loss of neurons in unsupplemented controls. The surviving cells expressed trkB, the high-affinity receptor for BDNF. In contrast, neither nervegrowth factor nor neurotrophic factor 3 enhanced neuronal survival. Thus, BDNF supports the survival of neurons produced by the adult rat forebrain and may act as a permissive factor for neuronal recruitment in adulthood.
机译：神经元前体细胞持续存在于成年前脑室管膜/后胸膜区（SZ）中，并且已发现其在源自鸟类，啮齿动物和人类的培养物中产生神经元。我们推测这些细胞产生的神经元的存活可能在成年期受到局部缺乏营养支持的限制。为了验证该假设，我们建立了成年大鼠前脑SZ的外植体培养物，并评估了定义的神经营养蛋白对这些外植体产生的新神经元存活的影响。我们发现，微管相关蛋白2+神经元是由来自SZ广泛区域的外植体产生的，跨越了心室系统的前部6 mm。在暴露于脑源性神经营养因子（BDNF）的培养物中，> 35％的新神经元在体外22天（DIV）存活，而> 25％的患者在42 DIV存活，同时在未补充的对照组中神经元几乎完全丧失。存活的细胞表达trkB，即BDNF的高亲和力受体。相反，神经生长因子和神经营养因子3均未增强神经元存活率。因此，BDNF支持成年大鼠前脑产生的神经元的存活，并且可以作为成年期神经元募集的允许因素。

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