Human immunodeficiency virus (HIV) antigens: structure and serology of multivalent human mucin MUC1-HIV V3 chimeric proteins.

Fontenot JD; Gatewood JM; Mariappan SV; Pau CP; Parekh BS; George JR; Gupta G

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Human immunodeficiency virus (HIV) antigens: structure and serology of multivalent human mucin MUC1-HIV V3 chimeric proteins.

机译：人类免疫缺陷病毒（HIV）抗原：多价人类粘蛋白MUC1-HIV V3嵌合蛋白的结构和血清学。

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Molecular modeling and two-dimensional NMR techniques enable us to identify structural features in the third variable region (V3) loop of the human immunodeficiency virus (HIV) surface glycoprotein gp120, in particular the principal neutralizing determinant (PND), that remain conserved despite the sequence variation. The conserved structure of the PND is a solvent-accessible protruding motif or a knob, structurally isomorphous with the immunodominant knobs in the tandem repeat protein of human mucin 1 (MUC1) (a tumor antigen for breast, pancreatic, and ovarian cancer). We have replaced the mucin antigenic knobs by the PND knobs of the HIV MN isolate in a set of chimeric human MUC1/HIV V3 antigens. This produced multivalent HIV antigens in which PNDs are located at regular intervals and separated by extended mucin spacers. In this article we show by two-dimensional NMR spectroscopy that the multivalent antigens preserve the PNDs in their native structure. We also demonstrate by ELISA that the antigens correctly present the PNDs for binding to monoclonal antibodies or polyclonal antisera from HIV-infected patients.

机译：分子建模和二维NMR技术使我们能够鉴定人免疫缺陷病毒（HIV）表面糖蛋白gp120的第三可变区（V3）环中的结构特征，尤其是主要中和决定簇（PND），尽管序列变异。 PND的保守结构是溶剂可及的突出基序或纽结，与人粘蛋白1（MUC1）（乳腺癌，胰腺癌和卵巢癌的肿瘤抗原）的串联重复蛋白中的免疫优势纽结在结构上同构。我们已经用一组嵌合人类MUC1 / HIV V3抗原中的HIV MN分离物的PND旋钮代替了粘蛋白抗原旋钮。这产生了多价的HIV抗原，其中PND以规则的间隔定位，并被延伸的粘蛋白间隔子隔开。在本文中，我们通过二维NMR光谱表明，多价抗原将PND保留在其天然结构中。我们还通过ELISA证明了抗原正确地呈现了与来自HIV感染患者的单克隆抗体或多克隆抗血清结合的PND。

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来源
《Proceedings of the National Academy of Sciences of the United States of America》 |1995年第1期|P.315-319|共5页
作者
Fontenot JD; Gatewood JM; Mariappan SV; Pau CP; Parekh BS; George JR; Gupta G;
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作者单位

Life Sciences Division, Los Alamos National Laboratory, NM 87545.;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
Antibodies; Monoclonal; HIV Antibodies; HIV Antigens; Membrane Glycoproteins; 抗体; 单克隆; HIV抗体; HIV抗原; 膜糖蛋白类; 粘蛋白类; 肽类; 蛋白质结构; 二级;

机译：Antibodies;Monoclonal;HIV Antibodies;HIV Antigens;Membrane Glycoproteins;抗体;单克隆;HIV抗体;HIV抗原;膜糖蛋白类;粘蛋白类;肽类;蛋白质结构;二级;

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1. Replacement of the V3 domain in the surface subunit of the feline immunodeficiency virus envelope glycoprotein with the equivalent region of a T cell-tropic human immunodeficiency virus type 1 results in a chimeric surface protein that efficiently binds to CXCR4 [J] . GonzálezS.A., FalcónJ.I., AffranchinoJ.L. AIDS Research and Human Retroviruses . 2014,第3期

机译：猫免疫缺陷病毒包膜糖蛋白表面亚基中的V3结构域被1型T细胞嗜性人类免疫缺陷病毒的等效区域取代，从而形成了一种能有效结合CXCR4的嵌合表面蛋白
2. Replacement of the V3 domain in the surface subunit of the feline immunodeficiency virus envelope glycoprotein with the equivalent region of a T cell-tropic human immunodeficiency virus type 1 results in a chimeric surface protein that efficiently binds to CXCR4 [J] . GonzálezS.A., FalcónJ.I., AffranchinoJ.L. AIDS Research and Human Retroviruses . 2014,第3期

机译：用T细胞 - 热带人免疫缺陷病毒类型1的等同区域替换翅膀免疫缺陷病毒包膜糖蛋白的表面亚基中的V3结构域导致嵌合表面蛋白有效结合CXCR4
3. Construction and infection of a new simian/human immunodeficiency chimeric virus (SHIV) containing the integrase gene of the human immunodeficiency virus type 1 genome and analysis of its adaptation to monkey cells. [J] . Akiyama H, Ishimatsu M, Miura T, Microbes and infection . 2008,第5期

机译：新型猿猴/人类免疫缺陷病毒嵌合病毒（SHIV）的构建和感染，该病毒包含1型人类免疫缺陷病毒基因组的整合酶基因，并分析了其对猴细胞的适应性。
4. Simultaneous extraction of human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) RNA with the M1000 apparatus does not impair the measurement of the viral load of both viruses when tested by using the LCx PCR system [C] . T. Bourlet, S. Thamm, S. Pillet, European Congress of Virology . 2004

机译：通过使用LCX PCR系统测试，同时提取人免疫缺陷病毒类型1（HIV-1）和丙型肝炎病毒（HCV）RNA，不会损害两种病毒的病毒载量的测量
5. The role of the transmembrane and cytoplasmic domains of the VPU protein of human immunodeficiency virus type 1 (HIV-1) in the acute CD4+ T cell loss and disease in the simian human immunodeficiency virus (SHIV)/macaque model. [D] . Hout, David Richard. 2006

机译：人类免疫缺陷病毒1型（HIV-1）的VPU蛋白的跨膜和胞质域在猿猴人类免疫缺陷病毒（SHIV）/猕猴模型的急性CD4 + T细胞丧失和疾病中的作用。
6. Human immunodeficiency virus (HIV) antigens: structure and serology of multivalent human mucin MUC1-HIV V3 chimeric proteins. [O] . J D Fontenot, J M Gatewood, S V Mariappan, 1995

机译：人类免疫缺陷病毒（HIV）抗原：多价人类粘蛋白MUC1-HIV V3嵌合蛋白的结构和血清学。
7. Human immunodeficiency virus (HIV) antigens: structure and serology of multivalent human mucin MUC1-HIV V3 chimeric proteins. [O] . Fontenot, J D, Gatewood, J M, Mariappan, S V, 1995

机译：人类免疫缺陷病毒（HIV）抗原：多价人类粘蛋白MUC1-HIV V3嵌合蛋白的结构和血清学。
8. Economic Consequences of HIV (Human Immunodeficiency Virus) Infection for the Medicaid and Medicare Programs: An Exploratory Study. A Report on a Literature Search and a Review of Selected Research on Human Immunodeficiency Virus (HIV) and Health Care Financing Issues Including Topical Bibliographies and a Consolidated Bibliography [R] . Rayhawk, M. , Baily, M. A. 1989

机译：HIV（人类免疫缺陷病毒）感染医疗补助和医疗保险计划的经济后果：一项探索性研究。文献检索报告及人体免疫机能丧失病毒（HIV）和医疗保健融资问题选择研究的回顾，包括主题书目和综合参考书目

Human immunodeficiency virus (HIV) antigens: structure and serology of multivalent human mucin MUC1-HIV V3 chimeric proteins.

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