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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes.
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Disruption of the nucleoporin gene NUP133 results in clustering of nuclear pore complexes.

机译：核孔蛋白基因NUP133的破坏导致核孔复合物聚集。

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摘要

We have characterized a protein with an estimated molecular mass of 130 kDa that is contained in a highly enriched yeast nuclear pore complex (NPC) fraction. Partial amino acid sequence from this protein has led us to a previously identified open reading frame on chromosome XI of Saccharomyces cerevisiae encoding a protein of 133 kDa. Due to its coenrichment with NPCs during cell fractionation and the phenotype observed in the disrupted strain, we propose to term the gene encoding this protein NUP133. Cells carrying a disrupted copy of NUP133 were temperature sensitive for growth. In addition, abnormal clustering of NPCs was observed. This phenotype is similar to that previously observed in the disruption of another nucleoporin gene, NUP145. We speculate that the gene product of NUP133, Nup133p, may functionally overlap with the NUP145 gene product, Nup145p, and that these proteins may be involved in maintaining the position of the NPC within the nuclear envelope.
机译：我们已经鉴定出一种蛋白质，其估计分子量为130 kDa，包含在高度富集的酵母核孔复合物（NPC）馏分中。来自该蛋白质的部分氨基酸序列已使我们找到了先前鉴定的酿酒酵母XI染色体上的开放阅读框，其编码133 kDa的蛋白质。由于其在细胞分级分离过程中与NPCs共富集以及在破坏菌株中观察到的表型，我们建议对编码该蛋白NUP133的基因进行命名。携带NUP133复制副本的细胞对温度敏感。另外，观察到NPC的异常聚集。此表型类似于先前在破坏另一个核孔蛋白基因NUP145中观察到的表型。我们推测，NUP133的基因产物Nup133p可能与NUP145的基因产物Nup145p在功能上重叠，并且这些蛋白可能参与维持NPC在核膜中的位置。

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