...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Synthesis of a thymidyl pentamer of deoxyribonucleic guanidine and binding studies with DNA homopolynucleotides.
【24h】

Synthesis of a thymidyl pentamer of deoxyribonucleic guanidine and binding studies with DNA homopolynucleotides.

机译：脱氧核糖核酸胍的胸苷五聚体的合成和与DNA同多核苷酸的结合研究。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Replacement of the phosphodiester linkages of the polyanion DNA with guanidine linkers provides the polycation deoxynucleic guanidine (DNG). The synthesis of pentameric thymidyl DNA is provided. This polycationic DNG species binds with unprecedented affinity and with base-pair specificity to negatively charged poly(dA) to provide both double and triple helices. The dramatic stability of these hybrid structures is shown by their denaturation temperatures (Tm). For example, the double helix of the pentameric thymidyl DNG and poly(dA) does not dissociate in boiling water (ionic strength = 0.12), whereas the Tm for pentameric thymidyl DNA associated with poly(dA) is approximately 13 degrees C (ionic strength = 0.12). The effect of ionic strength on Tm for DNG complexes with DNA shows an opposite correlation compared with double-stranded DNA and is much more dramatic than for double-stranded DNA.
机译：用胍基连接基取代聚阴离子DNA的磷酸二酯键提供了聚阳离子脱氧核苷胍（DNG）。提供了五聚体胸苷基DNA的合成。这种聚阳离子DNG物质以前所未有的亲和力和碱基对特异性与带负电荷的poly（dA）结合，从而提供了双螺旋和三螺旋。这些杂化结构的变性稳定性由其变性温度（Tm）表示。例如，五聚胸苷DNG和poly（dA）的双螺旋在沸水中不会解离（离子强度= 0.12），而与poly（dA）相关的五聚胸苷DNA的Tm约为13摄氏度（离子强度= 0.12）。离子强度对具有DNA的DNG复合物的Tm的影响与双链DNA相比显示出相反的相关性，并且比双链DNA更为显着。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号