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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A corticosteroid-induced gene expressing an 'IsK-like' K+ channel activity in Xenopus oocytes.
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A corticosteroid-induced gene expressing an 'IsK-like' K+ channel activity in Xenopus oocytes.

机译：皮质类固醇诱导的基因在非洲爪蟾卵母细胞中表达“ IsK样” K +通道活性。

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摘要

Screening a rat colon cDNA library for aldosterone-induced genes resulted in the molecular cloning of a cDNA whose corresponding mRNA is strongly induced in the colon by dexamethasone, aldosterone, and a low NaCl diet. A similar mRNA was detected in kidney papilla but not in brain, heart, or skeletal muscle. Xenopus laevis oocytes injected with cRNA synthesized from this clone, designated CHIF (channel-inducing factor), express a K(+)-specific channel activity. The biophysical, pharmacological, and regulatory characteristics of this channel are very similar to those reported before for IsK (minK). These include: slow (tau > 20 s) activation by membrane depolarization with a threshold potential above -50 mV, blockade by clofilium, inhibition by phorbol ester, and activation by 8-bromoadenosine 3',5'-cyclic monophosphate and high cytoplasmic Ca2+. The primary structure of this clone, however, shows no homology to IsK. Instead, CHIF exhibits > 50% similarity to two other short bitopic membrane proteins, phospholemman and the gamma subunit of Na+K(+)-ATPase. The data are consistent with the possibility that CHIF is a member of a family of transmembrane regulators capable of activating endogenous oocyte transport proteins.
机译：筛选大鼠结肠cDNA文库中醛固酮诱导的基因导致分子的克隆，该cDNA的相应mRNA被地塞米松，醛固酮和低NaCl饮食强烈诱导到结肠中。在肾乳头中检测到类似的mRNA，但在脑，心脏或骨骼肌中未检测到。非洲爪蟾卵母细胞注射从此克隆合成的cRNA，称为CHIF（通道诱导因子），表达K（+）特异性通道活性。该通道的生物物理，药理学和调节特性与之前报道的IsK（minK）非常相似。其中包括：通过膜去极化（阈值电位高于-50 mV）缓慢激活（tau> 20 s），被clofilium阻断，被佛波酯抑制以及被8-溴腺苷3'，5'-环一磷酸和高细胞质Ca2 +激活。 。但是，该克隆的一级结构与IsK没有同源性。取而代之的是，CHIF与另两个短的双向位膜蛋白，磷酸化lemman和Na + K（+）-ATPase的γ亚基具有> 50％的相似性。数据与CHIF是能够激活内源卵母细胞转运蛋白的跨膜调节剂家族成员的可能性一致。

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