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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Precursor of C4 antisense RNA of bacteriophages P1 and P7 is a substrate for RNase P of Escherichia coli.
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Precursor of C4 antisense RNA of bacteriophages P1 and P7 is a substrate for RNase P of Escherichia coli.

机译：噬菌体P1和P7的C4反义RNA的前体是大肠杆菌RNase P的底物。

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摘要

The C4 repressor of the temperate bacteriophages P1 and P7 inhibits antirepressor (Ant) synthesis and is essential for establishment and maintenance of lysogeny. C4 is an antisense RNA acting on a target, Ant mRNA, which is transcribed from the same promoter. The antisense-target RNA interaction requires processing of C4 RNA from a precursor RNA. Here we show that 5' maturation of C4 RNA in vivo depends on RNase P. In vitro, Escherichia coli RNase P and its catalytic RNA subunit (M1 RNA) can generate the mature 5' end of C4 RNA from P1 by a single endonucleolytic cut, whereas RNase P from the E. coli rnpA49 mutant, carrying a missense mutation in the RNase P protein subunit, is defective in the 5' maturation of C4 RNA. Primer extension analysis of RNA transcribed in vivo from a plasmid carrying the P1 c4 gene revealed that 5'-mature C4 RNA was the predominant species in rnpA+ bacteria, whereas virtually no mature C4 RNA was found in the temperature-sensitive rnpA49 strain at the restrictive temperature. Instead, C4 RNA molecules carrying up to five extra nucleotides beyond the 5' end accumulated. The same phenotype was observed in rnpA+ bacteria which harbored a plasmid carrying a P7 c4 mutant gene with a single C-->G base substitution in the structural homologue to the CCA 3' end of tRNAs. Implications of C4 RNA processing for the lysis/lysogeny decision process of bacteriophages P1 and P7 are discussed.
机译：温带噬菌体P1和P7的C4阻遏物抑制抗阻遏物（Ant）的合成，对于建立和维持溶原性至关重要。 C4是作用于靶标Ant mRNA的反义RNA，可从同一启动子转录而来。反义-靶标RNA相互作用需要来自前体RNA的C4RNA的加工。在这里，我们显示体内C4 RNA的5'成熟取决于RNaseP。在体外，大肠杆菌RNase P及其催化RNA亚基（M1 RNA）可以通过一次内切核酸酶切从P1生成C4 RNA的成熟5'末端，而来自大肠杆菌rnpA49突变体的RNase P在RNase P蛋白亚基中带有错义突变，则在C4 RNA的5'成熟中存在缺陷。从携带P1 c4基因的质粒体内转录的RNA的引物延伸分析表明，在rnpA +细菌中5'-成熟C4 RNA是主要种类，而在温度敏感的rnpA49菌株中，在限制性酶切细菌中实际上未发现成熟的C4 RNA。温度。取而代之的是，C5 RNA分子会在5'端以外携带多达五个额外的核苷酸。在rnpA +细菌中观察到了相同的表型，该细菌带有一个质粒，该质粒带有一个p7 c4突变基因，该基因在与tRNA的CCA 3'端的结构同源物中具有单个C→G碱基取代。讨论了C4 RNA处理对噬菌体P1和P7的裂解/溶原性决定过程的影响。

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