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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >CD4(+) BLOOD LYMPHOCYTES ARE RAPIDLY KILLED IN VITRO BY CONTACT WITH AUTOLOGOUS HUMAN IMMUNODEFICIENCY VIRUS-INFECTED CELLS
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CD4(+) BLOOD LYMPHOCYTES ARE RAPIDLY KILLED IN VITRO BY CONTACT WITH AUTOLOGOUS HUMAN IMMUNODEFICIENCY VIRUS-INFECTED CELLS

机译：通过与自动徽标人类免疫缺陷病毒感染的细胞接触，可快速杀死CD4（+）血液淋巴细胞

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摘要

We have investigated the ability of human immunodeficiency virus (HIV)-infected cells to kill uninfected CD4(+) lymphocytes. Infected peripheral blood mononuclear cells were cocultured with autologous Cr-51-labeled uninfected cells. Rapid death of the normal CD4-expressing target population was observed following a brief incubation. Death of blood CD4(+) lymphocytes occurred before syncytium formation could be detected or productive viral infection established in the normal target cells. Cytolysis could not be induced by free virus, was dependent on gp120-CD4 binding, and occurred in resting, as well as activated, lymphocytes. CD8(+) cells were not involved in this phenomenon, since HIV-infected CEMT4 cells (CD4(+), CD8(-) cells) mediated the cytolysis of uninfected targets. Reciprocal isotope-labeling experiments demonstrated that infected CEMT4 cells did not die in parallel with their targets. The uninfected target cells manifested DNA fragmentation, followed by the release of the Cr-51 label. Thus, in HIV patients, infected lymphocytes may cause the depletion of the much larger population of uninfected CD4(+) cells without actually infecting them, by triggering an apoptotic death. [References: 34]
机译：我们已经调查了人类免疫缺陷病毒（HIV）感染的细胞杀死未感染的CD4（+）淋巴细胞的能力。将感染的外周血单核细胞与自体Cr-51标记的未感染细胞共培养。短暂孵育后，观察到正常表达CD4的目标人群迅速死亡。在可以检测到合胞体形成或正常靶细胞中建立了生产性病毒感染之前，血液CD4（+）淋巴细胞已经死亡。游离病毒不能诱导细胞溶解，它取决于gp120-CD4的结合，并发生在静止的以及活化的淋巴细胞中。 CD8（+）细胞不参与此现象，因为HIV感染的CEMT4细胞（CD4（+），CD8（-）细胞）介导了未感染靶标的细胞溶解。相互同位素标记实验表明，感染的CEMT4细胞不会与其靶标平行死亡。未感染的靶细胞表现出DNA断裂，然后释放Cr-51标记。因此，在HIV患者中，受感染的淋巴细胞可能会触发凋亡性死亡，从而导致大量未感染的CD4（+）细胞耗尽，而实际上并未感染它们。 [参考：34]

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