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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >STRUCTURAL ORGANIZATION OF MOUSE PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA (MPPAR-GAMMA) GENE - ALTERNATIVE PROMOTER USE AND DIFFERENT SPLICING YIELD TWO MPPAR-GAMMA ISOFORMS
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STRUCTURAL ORGANIZATION OF MOUSE PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA (MPPAR-GAMMA) GENE - ALTERNATIVE PROMOTER USE AND DIFFERENT SPLICING YIELD TWO MPPAR-GAMMA ISOFORMS

机译：小鼠过氧化物酶增殖剂活化受体γ（MPPAR-γ）基因的结构组织-交替使用促进子和两种MPPAR-γ异构体的拼接。

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To gain insight into the regulation of expression of peroxisome proliferator-activated receptor (PPAR) isoforms, we have determined the structural organization of the mouse PPAR gamma (mPPAR gamma) gene, This gene extends >105 kb and gives rise to two mRNAs (mPPAR gamma 1 and mPPAR gamma 2) that differ at their 5' ends, The mPPAR gamma 2 cDNA encodes an additional 30 amino acids N-terminal to the first ATG codon of mPPAR gamma 1 and reveals a different 5' untranslated sequence, We show that mPPAR gamma 1 mRNA is encoded by eight exons, whereas the mPPAR gamma 2 mRNA is encoded by seven exons, Most of the 5' untranslated sequence of mPPAR gamma 1 mRNA is encoded by two exons, whereas the 5' untranslated sequence and the extra 30 N-terminal amino acids of mPPAR gamma 2 are encoded by one exon, which is located between the second and third exons coding for mPPAR gamma 1, The last six exons of mPPAR gamma gene code for identical sequences in mPPAR gamma 1 and mPPAR gamma 2 isoforms, The mPPAR gamma 1 and mPPAR gamma 2 isoforms are transcribed from different promoters, The mPPAR gamma gene has been mapped to chromosome 6 E3-F1 by in situ hybridization using a biotin-labeled probe. These results establish that at least one of the PPAR genes yields more than one protein product, similar to that encountered with retinoid X receptor and retinoic acid receptor genes. The existence of multiple PPAR isoforms transcribed from different promoters could increase the diversity of ligand and tissue-specific transcriptional responses. [References: 22]
机译：为了深入了解过氧化物酶体增殖物激活受体（PPAR）亚型的表达调控，我们确定了小鼠PPAR gamma（mPPAR gamma）基因的结构组织，该基因延伸> 105 kb，并产生两个mRNA（mPPAR γ1和mPPARγ2）在其5'末端不同，mPPARγ2 cDNA在mPPARγ1的第一个ATG密码子N端编码了另外30个氨基酸，并揭示了不同的5'非翻译序列，我们证明了mPPAR gamma 1 mRNA由八个外显子编码，而mPPAR gamma 2 mRNA由七个外显子编码，mPPAR gamma 1 mRNA的大多数5'非翻译序列由两个外显子编码，而5'非翻译序列和另外30个外显子编码mPPARγ2的N末端氨基酸由一个外显子编码，该外显子位于编码mPPARγ1的第二和第三外显子之间。mPPARγ基因的最后六个外显子编码mPPARγ1和mPPARγ2中的相同序列。异构体PARγ1和mPPARγ2亚型从不同的启动子转录而来。mPPARγ基因已通过生物素标记探针通过原位杂交定位到6 E3-F1染色体上。这些结果证明至少一个PPAR基因产生一种以上的蛋白质产物，这与类维生素A X受体和视黄酸受体基因相似。从不同启动子转录的多个PPAR亚型的存在可能会增加配体和组织特异性转录反应的多样性。 [参考：22]

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