...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >TARGETED GENE INACTIVATION IN PETUNIA BY PCR-BASED SELECTION OF TRANSPOSON INSERTION MUTANTS
【24h】

TARGETED GENE INACTIVATION IN PETUNIA BY PCR-BASED SELECTION OF TRANSPOSON INSERTION MUTANTS

机译：基于PCR的转座子插入突变体选择在靶基因中的目的基因灭活

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Establishment of loss-of-function pheno-types is often a key step in determining the biological function of a gene. We describe a procedure to obtain mutant petunia plants in which a specific gene with known sequence is inactivated by the transposable element dTphl. Leaves are collected from batches of 1000 plants with highly active dTphl elements, pooled according to a three-dimensional matrix, and screened by PCR using a transposon- and a gene-specific primer. In this way individual plants with a dTphl insertion can be identified by analysis of about 30 PCRs. We found insertion alleles for various genes at a frequency of about 1 in 1000 plants. The plant population can be preserved by selfing all the plants, so that it can be screened for insertions in many genes over a prolonged period. [References: 36]
机译：功能丧失表型的建立通常是确定基因生物学功能的关键步骤。我们描述了一种获得突变矮牵牛植物的程序，其中具有已知序列的特定基因被转座因子dTphl灭活。从具有高活性dTphl元素的1000批植物的批次中收集叶子，根据三维矩阵合并，并使用转座子和基因特异性引物通过PCR进行筛选。以此方式，可以通过分析约30个PCR来鉴定具有dTph1插入的单个植物。我们发现了1000种植物中频率约为1的各种基因的插入等位基因。可以通过对所有植物进行自交保护来保留植物种群，从而可以长期筛选其是否插入了许多基因。 [参考：36]

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号