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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >IDENTIFICATION AND CHARACTERIZATION OF PUTATIVE TRANSPOSABLE DNA ELEMENTS IN SOLANACEOUS PLANTS AND CAENORHABDITIS ELEGANS
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IDENTIFICATION AND CHARACTERIZATION OF PUTATIVE TRANSPOSABLE DNA ELEMENTS IN SOLANACEOUS PLANTS AND CAENORHABDITIS ELEGANS

机译：茄科植物和拟南芥中正负性可转移DNA元素的鉴定和表征

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摘要

Several families of putative transposable elements (TrEs) in both solanaceous plants and Caenorhabditis elegans have been identified by screening the DNA data base for inverted repeated domains present in multiple copies in the genome, The elements are localized within intron and flanking regions of many genes, These elements consist of two inverted repeats flanking sequences ranging from 5 bp to >500 bp, Identification of multiple elements in which sequence conservation includes both the flanking and internal regions implies that these TrEs are capable of duplicative transposition, Two of the elements were identified in promoter regions of the tomato (Lycopersicon esculentum) polygalacturonase and potato (Solanum tuberosum) Win1 genes, The element in the polygalacturonase promoter spans a known regulatory region. In both cases, ancestral DNA sequences, which represent potential recombination target sequences prior to insertion of the elements, have been cloned from related species, The sequences of the inverted repeated domains in plants and C, elegans show a high degree of phylogenetic conservation, While frequency of the different elements is variable, some are present in very high copy number, A member of a single C, elegans TrE family is observed approximately once every 20 kb in the genome, The abundance of the described TrEs suggests utility in the genomic analysis of these and related organisms. [References: 24]
机译：通过筛选DNA数据库中基因组多个副本中存在的反向重复结构域，已经鉴定了茄科植物和秀丽隐杆线虫中的几种推定转座因子（TrE）家族。这些元素位于许多基因的内含子和侧翼区域，这些元件由5个bp至> 500 bp范围内的两个反向重复序列侧翼序列组成。鉴定出多个元素，其中序列保守性包括侧翼和内部区域，意味着这些TrE能够复制转座。番茄（Lycopersicon esculentum）聚半乳糖醛酸酶和马铃薯（Solanum tuberosum）Win1基因的启动子区域。聚半乳糖醛酸酶启动子中的元件跨越一个已知的调控区域。在这两种情况下，均已从相关物种中克隆了代表潜在的重组靶序列的祖先DNA序列，该序列是从相关物种中克隆的。不同元件的频率是可变的，一些以很高的拷贝数存在，在基因组中大约每20 kb观察到一个C秀丽隐杆线虫TrE家族的成员，所述TrE的丰度表明在基因组分析中的用途这些和相关生物。 [参考：24]

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