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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >GUT-SPECIFIC TRANSCRIPTIONAL REGULATORY ELEMENTS OF THE CARBOXYPEPTIDASE GENE ARE CONSERVED BETWEEN BLACK FLIES AND DROSOPHILA
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GUT-SPECIFIC TRANSCRIPTIONAL REGULATORY ELEMENTS OF THE CARBOXYPEPTIDASE GENE ARE CONSERVED BETWEEN BLACK FLIES AND DROSOPHILA

机译：羧肽酶基因的肠道特异性转录调节元件在黑蝇和果蝇之间是保守的

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摘要

Millions of people die every year in the tropical world from diseases transmitted by hematophagous insects. Failure of conventional containment measures emphasizes the need for additional approaches, such as transformation of vector insects with genes that restrict vectorial capacity. The availability of an efficient promoter to drive foreign genes in transgenic insects is a necessary tool to test the feasibility of such approach. Here we characterize the putative promoter region of a black fly midgut carboxypeptidase gene and show that these sequences correctly direct the expression of a beta-glucuronidase reporter in Drosophila melanogaster. By histochemical staining and mRNA analysis, we found that the gene is expressed strongly and gut-specifically in the transgenic Drosophila. This gut-specific black fly carboxypeptidase promoter provides a valuable tool for the study of disease vectors. [References: 23]
机译：在热带世界，每年有数百万人死于食血昆虫传播的疾病。传统收容措施的失败强调了对其他方法的需求，例如用限制载体能力的基因转化媒介昆虫。有效的启动子来驱动转基因昆虫中的外源基因是测试这种方法可行性的必要工具。在这里，我们表征黑蝇中肠羧肽酶基因的推定启动子区域，并表明这些序列正确地指导果蝇中果蝇β-葡萄糖醛酸酶报道基因的表达。通过组织化学染色和mRNA分析，我们发现该基因在果蝇的转基因果蝇中强烈和肠道特异性表达。这种肠道特异性的黑蝇羧肽酶启动子为研究疾病载体提供了有价值的工具。 [参考：23]

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