...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Accelerated chemical synthesis of peptides and small proteins
【24h】

Accelerated chemical synthesis of peptides and small proteins

机译：加速化学合成肽和小蛋白质

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The chemical synthesis of peptides and small protecins is a powerful complementary strategy to recombinant Protein overexpression and is widely used in structural biology, immunology, protein engineering, and biomedical research. De- spite considerable improvements in the fidelity of peptide chain assembly, side-chain protection, and postsynthesis analysis, a limiting factor in accessing polypeptides containing greater than 50 residues remains the time taken for chain assembly. The ultimate goal of this work is to establish highly efficient chemical procedures that achieve chain-assembly rates of approximately 10-15 residues per hour, thus underpinning the rapid chemical synthesis of long polypeptides and proteins, including cyto- kines, growth factors, protein domains, and small enzymes. Here we report Boc chemistry that employs O-(7-azabenzo- triazol-I-yl) -N,N,N',N' -tetramethyluronium hexafluorOphOs- phate (HATU)/dimethyl sulfoxide in situ neutralization as the coupling agent and incorporates a protected amino acid residuc every 5 min to produce peptides of good quality. This rapid coupling chemistry was successfully demonstrated by synthesiz- ing several small to medium peptides, including the "difficult'' C-terminal sequence of HIV-1 proteinase (residues 81-99); frag- ment 65-74 of the acyl carrier protein; conotoxin PnIA(AIOL), a potent neuronal nicotinic receptor antagonist: and the pro- inflammatory chemotactic protein CP10, an 88-residue protein, by means of native chemical ligation. The benefits of this approach include enhanced ability to identify and charactcri
机译：肽和小蛋白的化学合成是重组蛋白过表达的有力补充策略，已广泛用于结构生物学，免疫学，蛋白工程和生物医学研究中。尽管在肽链装配的保真度，侧链保护和合成后分析方面有相当大的改进，但访问包含大于50个残基的多肽的限制因素仍然是链装配所花费的时间。这项工作的最终目标是建立高效的化学程序，以达到每小时约10-15个残基的链组装速率，从而支持长多肽和蛋白质（包括细胞因子，生长因子，蛋白质结构域）的快速化学合成。和小酵素。在这里，我们报告Boc化学，它采用O-（7-氮杂苯并三唑-I-基）-N，N，N'，N'-四甲基O六氟膦（HATU）/二甲基亚砜原位中和作为偶联剂并结合每5分钟会还原受保护的氨基酸，以生产出高质量的多肽。通过合成几种中小型肽，包括HIV-1蛋白酶的“困难” C端序列（残基81-99）；酰基载体蛋白的残基65-74，成功地证明了这种快速偶联化学；一种有力的神经元烟碱受体拮抗剂； conotoxin PnIA（AIOL）；以及一种通过天然化学连接的促炎性趋化蛋白CP10（一种88个残基的蛋白），这种方法的好处包括增强了识别和鉴定特征的能力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号