...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >G beta 5 prevents the RGS7-G alpha o interaction through binding to a distinct G gamma-like domain found in RGS7 and other RGS proteins
【24h】

G beta 5 prevents the RGS7-G alpha o interaction through binding to a distinct G gamma-like domain found in RGS7 and other RGS proteins

机译：G beta 5通过与RGS7和其他RGS蛋白中发现的独特的Gγ样结构域结合来阻止RGS7-G alpha o相互作用

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The G protein beta subunit G beta 5 deviates significantly from the other four members of G beta-subunit family in amino acid sequence and subcellular localization. To detect the protein targets of G beta 5 in vivo, we have isolated a native G beta 5 protein complex from the retinal cytosolic fraction and identified the protein tightly associated with G beta 5 as the regulator of G protein signaling (RGS) protein, RGS7. Here we show that complexes of G beta 5 with RGS protcins can be formed in vitro from the recombinant proteins. The reconstituted G beta 5-RGS dimers are similar to thc native retinal complex in their behavior on gel-filtration and cation- exchange chromatographics and can be immunoprecipitated with either anti-GP5 or anti-RGS7 antibodies. The specific G beta 5-RGS7 interaction is determined by a distinct domain in RGS that has a striking homology to Gr subunits. Deletion of this domain prevents the RGS7-GP5 binding, although the interaction with G alpha is retained. Substitution of the G r-like domain of RGS7 with a portion of G r1 changes its binding specificity from G beta 5 to Gbeta 1. The interaction of G beta 5 with RGS7 blocked the binding of RGS7 to the G alpha subunit G alpha o, indicating that G beta 5 is a specific RGS inhibitor.
机译：G蛋白beta亚基G beta 5在氨基酸序列和亚细胞定位方面与G beta亚基家族的其他四个成员明显不同。为了在体内检测G beta 5的蛋白质​​靶标，我们从视网膜细胞溶质组分中分离出了天然G beta 5蛋白质复合物，并确定了与G beta 5紧密相关的蛋白质是G蛋白信号（RGS）蛋白质RGS7的调节剂。 。在这里，我们显示可以从重组蛋白体外形成G beta 5与RGS蛋白质的复合物。重构的Gβ5-RGS二聚体在凝胶过滤和阳离子交换色谱上的行为类似于天然视网膜复合物，并且可以用抗GP5或抗RGS7抗体进行免疫沉淀。特定的Gβ5-RGS7相互作用是由RGS中与Gr亚基具有惊人同源性的独特域决定的。尽管保留了与G alpha的相互作用，但该结构域的缺失阻止了RGS7-GP5的结合。用一部分G r1取代RGS7的类似G r的结构域，将其结合特异性从G beta 5变为Gbeta1。Gbeta 5与RGS7的相互作用阻止了RGS7与G alpha亚基G alpha o的结合，表明G beta 5是特定的RGS抑制剂。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号