...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of the block in targeted retroviral-mediated gene transfer
【24h】

Identification of the block in targeted retroviral-mediated gene transfer

机译：确定靶向逆转录病毒介导的基因转移中的障碍

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A chimeric retroviral vector (33E67) contain- ing a CD33-spocific single-chain antibody was generated in an attempt to target cells displaying the CD33 surface antigen. The chimeric envelope protein was translated, processed, and incor- porated into viral particles as efficiently as wild-type envelope protein. The viral particles carrying the 33E67 envelope protein could bind efficiently to the CD33 receptor on target cells and were internalized, but no gene transfer occurred. A unique experimental approach was used to examine the basis for this postbinding block. Our data indicate that the chimeric envelope protein itself cannot participate in the fusion process the most reasonablc cxplanation being that this chimeric protein cannot undergo the appropriate conformational change that is thought to be triggered by receptor binding, a suggested prerequisite to subsequent fosion and core entry. These results indicate that the hlock to gene transfer in this system, and probably in most of the current chimeric retroviral vectors to date, is the inability of the chimeric envelope protein to undergo this obligatory conforma- tional change.
机译：产生了一种含有CD33特异性单链抗体的嵌合逆转录病毒载体（33E67），试图靶向展示CD33表面抗原的细胞。嵌合包膜蛋白的翻译，加工和掺入病毒颗粒的效率与野生型包膜蛋白一样有效。携带33E67包膜蛋白的病毒颗粒可以有效结合靶细胞上的CD33受体并被内化，但没有发生基因转移。一种独特的实验方法被用来检查此后绑定块的基础。我们的数据表明，嵌合包膜蛋白本身不能参与融合过程，最合理的理由是该嵌合蛋白不能进行适当的构象变化，而这种构象变化被认为是由受体结合​​触发的，这是随后的先驱和核心进入的建议先决条件。这些结果表明，在该系统中以及迄今为止可能在大多数当前的嵌合逆转录病毒载体中，基因转移的障碍是嵌合包膜蛋白无法进行这种强制性构象变化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号