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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Patterning cells and their environments using multiple laminar fluid flows in capillary net-works
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Patterning cells and their environments using multiple laminar fluid flows in capillary net-works

机译：在毛细管网络中使用多个层流对细胞及其环境进行图案化

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摘要

This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cells adjacent to each other, the patterned delivery of chemicals to adhered cells, and performing enzymatic reactions over select cell or over a portion of a cell. This method offers a way to simultaneously control the characteristics of the surface to which cells are attached, the type of cells that are in their vicinity, and the kind of media that cells or part of a cell are exposed to. The method is experimentally Simple, highly adaptable, and requires no special equipment except for an elastomeric relief that can be readily prepared by rapid prototyping.
机译：本文介绍了在毛细管系统中使用层流液体来图案化细胞培养基质，执行图案化的细胞沉积以及图案化细胞培养基的方法。我们展示了具有不同蛋白质的细胞培养底物的图案，彼此相邻的不同类型的细胞的图案，化学物质向粘附细胞的图案化递送以及在选定的细胞或部分细胞上进行酶促反应。该方法提供了一种方法，可以同时控制附着细胞的表面的特性，附近细胞的类型以及细胞或细胞部分所暴露的介质的类型。该方法在实验上很简单，适应性强，除了可以通过快速成型容易制备的弹性浮雕外，不需要任何特殊设备。

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