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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Plant ribosome recycling factor homologne is a chloroplastic protein and is bactericidal in Escheritchia coli. carrying temperature-sensitive ribosome recycling factor
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Plant ribosome recycling factor homologne is a chloroplastic protein and is bactericidal in Escheritchia coli. carrying temperature-sensitive ribosome recycling factor

机译：植物核糖体回收因子同系物是一种叶绿体蛋白，在大肠杆菌中具有杀菌作用。携带温度敏感核糖体回收因子

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摘要

We have isolated a protein, mature RRFHCP, from chloroplasts of spinach (Spinacia ol'eracea L.) that shows 46 sequence identity and 66 sequcnce homology with ribosome recycling factor (RRF) of Escherichia coli. RRF recycles ribosomes through disassembly of the posttermination complex. From the cDNA analysis and from the amino-terminal sequencing of the isolated protein, the mature RRFHCP was deduced to have a Mr of 21,838 with 193 aa. It lacks the 78-aa chloroplast targeting sequence encoded by the RRFHCP cDNA sequence. The RRFHCP synthesized in viTRO was imported into isolated chloroplasts with simultaneous conversion to the mature RRF-HCP. Transcription of the gene coding for RRFHCP was not dependent on light, yet it was limited mostly to photosynthetic tissues in which only one transcript size was detected. Mature RRFHCP exerted a bactericidal effect on E. coli carrying temperature- sensitive RRF at the permissive temperature whereas wild-type E. coli was not affected.
机译：我们已经从菠菜（Spinacia ol'eracea L.）的叶绿体中分离出一种成熟的RRFHCP蛋白，该蛋白与大肠杆菌的核糖体回收因子（RRF）表现出46个序列同一性和66个序列同源性。 RRF通过拆卸终止复合物来回收核糖体。从cDNA分析和分离蛋白的氨基末端测序，推论出成熟的RRFHCP的Mr为21838，氨基酸为193aa。它缺少由RRFHCP cDNA序列编码的78-aa叶绿体靶向序列。体外合成的RRFHCP被导入分离的叶绿体中，同时转化为成熟的RRF-HCP。编码RRFHCP的基因的转录不依赖于光，但它主要限于仅检测到一个转录本大小的光合组织。成熟的RRFHCP在允许温度下携带对温度敏感的RRF对大肠杆菌产生杀菌作用，而野生型大肠杆菌则不受影响。

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