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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Aquaporin-6: An intracellular vesicle water channel protein in renal epithelia .
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Aquaporin-6: An intracellular vesicle water channel protein in renal epithelia .

机译：Aquaporin-6：肾上皮细胞内囊泡水通道蛋白。

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摘要

All characterized mammalian aquaporins (AQPs) are localized to plasma membranes where they function chiefly to mediate water transport across cells. Here we show that AQP6 is localized exclusively in intracellular membranes in renal epithelia. By using a polyclonal antibody to the C terminus of AQP6, immunoblots revealed a major 30-kDa band in membranes from rat renal cortex and medulla. Endoglycosidase treatment demonstrated presence of an in- tracellular high mannose glycan on each subunit. Sequential ultracentrifugation of rat kidney homogenates confirmed that AQP6 resides predominantly in vesicular fractions, and immunohistochemical and immunoelectron microscopic studies confirmed that >98 of AQP6 is located in intracellular membrane vesicles. In glomeruli, AQP6 is present in membrane vesicles within podocyte cell bodies and foot processes. In proximal tubules, AQP6 is also abundant in membrane vesicles within the subapical compartment of segment 2 and segment 3 cells, but was not detected in the brush border or basolateral membranes. In collecting duct, AQP6 resides in intracellular membrane vesicles in apical, mid, and basolatcral cytoplasm of type A intercalated cells, but was not observed in the plasma membrane. Unlike other members of the AQP family, the unique distribution in intracellular membrane vesicles in multiple types of renal epithelia indicates that AQP6 is not simply involved in transcellular fluid absorption. Moreover, our studies predict that AQP6 participates in distinct physiological functions such as glomerular filtration, tubular endocytosis, and
机译：所有表征的哺乳动物水通道蛋白（AQP）均定位于质膜，在质膜中它们主要起介导水跨细胞运输的作用。在这里，我们显示AQP6仅位于肾脏上皮细胞内膜中。通过使用针对AQP6 C末端的多克隆抗体，免疫印迹揭示了来自大鼠肾皮质和髓质膜的30 kDa主要条带。糖苷内切酶处理表明每个亚基上都存在细胞内高甘露糖聚糖。大鼠肾脏匀浆的连续超速离心证实AQP6主要存在于囊泡级分中，免疫组织化学和免疫电子显微镜研究证实，> 98的AQP6位于细胞内膜囊泡中。在肾小球中，AQP6存在于足细胞细胞体和足突内的膜囊泡中。在近端小管中，AQP6在第2段和第3段细胞的根尖隔室内的膜囊泡中也丰富，但在刷状缘或基底外侧膜中未检测到。在收集管道中，AQP6驻留在A型插层细胞的顶端，中部和杆状细胞质的细胞内膜囊泡中，但未在质膜中观察到。与AQP家族的其他成员不同，多种类型的肾上皮细胞在细胞内膜囊泡中的独特分布表明AQP6不仅仅参与跨细胞液的吸收。此外，我们的研究预测AQP6参与不同的生理功能，例如肾小球滤过，肾小管内吞和

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