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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Snt309p modulates interactions of Prp19p with its associated components to stabilize the Prp19p-associated complex essential for pre-mRNA splicing
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Snt309p modulates interactions of Prp19p with its associated components to stabilize the Prp19p-associated complex essential for pre-mRNA splicing

机译：Snt309p调节Prp19p及其相关成分的相互作用，以稳定mRNA前剪接必不可少的Prp19p相关复合物

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摘要

The SNT309 gene was identified via a mutation that causes lethality of cells in combination with a prplg mutation. We showed previously that Snt309p is a component of the PrpIgp-associatcd complex and that Snt309p, like Prplgp, is associated with the spliceosome immediately after or concomitantly with dissociation of U4 from the spliceosome. We show here that cxtracts prepared from the SNT309. deleted strain (ASNT309) were defective in splicing but could be complemented by addition of the purified Prplgp-associated complex. Isolation of the Prplgp-associated complex from ASNT309 extracts indicated that the complex was destabilized in the absence of Snt309p and dissociated on affinity chromatography, suggesting a role of Snt309p in stabilization of the Prp19p-associated complex. Addition of the affinity- purified Prplgp-Snt309p binary complex to ASNT309 extracts could reconstitute the Prp1gp-associated complex. Genetic analysis further suggests that Snt309p plays a role in modulating interactions of Prp19p with other associated components to facilitate formation of the Prp19p-associated complex. A model for how Snt309p modulates such interactions is proposed.
机译：SNT309基因是通过与prplg突变结合导致细胞致死的突变来鉴定的。先前我们已经证明Snt309p是PrpIgp相关复合体的组成部分，并且Snt309p与Prplgp一样，在U4从剪接体上解离后立即或伴随着剪接体。我们在这里显示从SNT309准备的cxtract。缺失的菌株（ASNT309）在剪接中有缺陷，但是可以通过添加纯化的Prplgp相关复合物来补充。从ASNT309提取物中分离出与Prplgp相关的复合物，表明该复合物在不存在Snt309p的情况下不稳定，并在亲和层析上解离，这表明Snt309p在稳定与Prp19p相关的复合物中的作用。将亲和纯化的Prplgp-Snt309p二元复合物添加到ASNT309提取物中可以重建Prp1gp相关的复合物。遗传分析进一步表明，Snt309p在调节Prp19p与其他相关组件的相互作用以促进Prp19p相关复合物的形成中发挥作用。提出了Snt309p如何调节此类相互作用的模型。

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