Kinase interaction domain of kinase-associated protein phosphatyase, a phosphoprotein-binding domain

JIA LI

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Kinase interaction domain of kinase-associated protein phosphatyase, a phosphoprotein-binding domain

机译：激酶相关蛋白磷酸酶的激酶相互作用域，一种磷酸蛋白结合域

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Kinase-associated protein phosphatase in teracts specifically with plant receptor-like protein kinases. This interation is thought to be a key step in signal perception and transduction. The minimal kinase interaction(ki) domain of kinase-associated protein phosphatase was mapped to a 119-aa segment spanning residues 180 t0 298. A forkhead-associated (FHA) homology region resides in this minimal KI domain. Site-directed mutagenesis of four highly conserved sites in this FHA homology region abolishes the KI domain's interaction with receptor-like protein kinases, indicating that the FHA region is esential for binding. Serial deletion analysis indicates that 30 aa on each side of the FHA region are also needed for binding; this minimal functional unit is designated as the KI domain. Kinetic studies using surface plasmon resonance indicate that the binding between the KI domain and receptor-like protein kinases has a dissociation constant (kd) of about 25-100 nM, which is similar to the binding affinity of two other well characterized phosphorylation-dependent protein-binding domains (14-3-3 and Src homology 2) and their high-affinity phosphopeptide ligands.

机译：激酶相关的蛋白磷酸酶特异与植物受体样蛋白激酶接触。这种相互作用被认为是信号感知和转导的关键步骤。激酶相关蛋白磷酸酶的最小激酶相互作用（ki）结构域定位到跨残基180 t0 298的119-aa区段。叉头相关（FHA）同源性区域位于此最小KI域中。在该FHA同源区域中四个高度保守的位点的定点诱变消除了KI域与受体样蛋白激酶的相互作用，表明FHA区对于结合至关重要。序列缺失分析表明，FHA区每侧需要30个氨基酸进行结合；该最小功能单元被称为KI域。使用表面等离振子共振的动力学研究表明，KI结构域与受体样蛋白激酶之间的结合具有约25-100 nM的解离常数（kd），这与其他两种表征良好的磷酸化依赖性蛋白的结合亲和力相似-结合结构域（14-3-3和Src同源性2）及其高亲和力磷酸肽配体。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1999年第14期|7821-7826|共6页
作者
JIA LI;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
biochemistrykinase inateraction domainproteins;

机译：生化激酶非作用域蛋白;

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1. iEKPD 2.0: an update with rich annotations for eukaryotic protein kinases, protein phosphatases and proteins containing phosphoprotein-binding domains [J] . Yaping Guo, Di Peng, Jiaqi Zhou, Nucleic acids research . 2019,第D1期

机译：iEKPD 2.0：带有丰富注释的真核蛋白激酶，蛋白磷酸酶和包含磷蛋白结合域的蛋白的更新
2. PepCyber:P～PEP: a database of human protein–protein interactions mediated by phosphoprotein-binding domains [J] . Ailing Hong, Dihan Zhou, Feifei Xiao, Nucleic acids research . 2008,第suppla1期

机译：PepCyber：P〜PEP：磷酸蛋白结合域介导的人蛋白相互作用的数据库
3. Phosphoprotein and phosphopeptide interactions with the FHA domain from Arabidopsis kinase-associated protein phosphatase. [J] . Ding Z, Wang H, Liang X, Biochemistry . 2007,第10期

机译：磷酸蛋白和磷酸肽与拟南芥激酶相关蛋白磷酸酶的FHA结构域相互作用。
4. HOW EFFECTIVE IS THE DATA ON CO-OCCURRENCE OF DOMAINS IN MULTI-DOMAIN PROTEINS IN PREDICTION OF PROTEIN-PROTEIN INTERACTIONS? [C] . Smita Mohanty, N. Srinivasan IEEE International Workshop on Genomic Signal Processing and Statistics . 2009

机译：在预测蛋白质 - 蛋白质相互作用中，多域蛋白在多域蛋白中的共同发生数据有何有效性？
5. The design and application of protein kinase-inducible domains as a general approach for detection of kinase and phosphatase activities. [D] . Gao, Feng. 2010

机译：蛋白激酶诱导型结构域的设计和应用作为检测激酶和磷酸酶活性的一般方法。
6. Kinase interaction domain of kinase-associated protein phosphatase a phosphoprotein-binding domain [O] . Jia Li, George P. Smith, John C. Walker 1999

机译：激酶相关蛋白磷酸酶的激酶相互作用域一种磷酸蛋白结合域
7. Kinase interaction domain of kinase-associated protein phosphatase, a phosphoprotein-binding domain [O] . Li, Jia, Smith, George P., Walker, John C. 1999

机译：激酶相关蛋白磷酸酶的激酶相互作用域，一种磷酸蛋白结合域

Kinase interaction domain of kinase-associated protein phosphatyase, a phosphoprotein-binding domain

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