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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The nop-1 gene of Neurospora crassa encodes a seven transmembrane helix retinal-binding protein homologous to archaeal rhodopsins
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The nop-1 gene of Neurospora crassa encodes a seven transmembrane helix retinal-binding protein homologous to archaeal rhodopsins

机译：Neurospora crassa的nop-1基因编码与古细菌视紫红质同源的七个跨膜螺旋视网膜结合蛋白

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摘要

Opsins are a class of retinal-binding, seven transmembrane helix proteins that function as light-responsive ion pumps or sensory receptors. Previously, genes encoding opsins had been identified in animals and the Archaea but not in fungi or other eukaryotic microorganisms. Here, we report the identification and mutational analysis of an opsin gene,nop-1, from the eukaryotic filamentous fungus neurospora crassa. The nop-1amino acid sequence predicts a protein that shares up to 81.8/100 amino acid identity with archaeal opsins in the 22 retinal binding pocket residues, including the conserved lysine residue that forms a Schiffbase linkage with retinal. Evolutionary analysis revealed related ness not only between NOP-1 and several fungal opsin-related proteins that lack the Schiff base lysine residue. The results provide evidence for a eukaryotic opsin family homologous to the archaeal opsins, providing a plausible link between archaeal and visual opsins. Extensive analysis of delta nop-1 strains did not reveal obvious defects in light-regulated processes under normal laboratory conditions. However, results from Northern analysis support light and condition-based regulation of nop-1 gene expression, and NOP-1 protein heterologously expressed in Pichia pastoris is labeled by using all-trans [3H] retinal, suggesting that NOP-1 functions as a rhodopsin in N.crassa photobiology.
机译：视蛋白是一类视网膜结合的七种跨膜螺旋蛋白，起光反应性离子泵或感觉受体的作用。以前，已在动物和古细菌中鉴定出编码视蛋白的基因，但在真菌或其他真核微生物中未鉴定到。在这里，我们报道了真核丝状真菌神经孢霉的视蛋白基因nop-1的鉴定和突变分析。 nop-1氨基酸序列预测该蛋白质与22个视网膜结合口袋残基中的古细菌视蛋白具有高达81.8 / 100个氨基酸同一性，其中包括与视网膜形成Schiffbase键的保守赖氨酸残基。进化分析显示，不仅NOP-1与几种缺乏席夫碱赖氨酸残基的真菌视蛋白相关蛋白之间具有相关性。结果提供了与古细菌视蛋白同源的真核视蛋白家族的证据，提供了古细菌视蛋白与视觉视蛋白之间的合理联系。在正常实验室条件下，对delta nop-1菌株的广泛分析未发现光调节过程中存在明显缺陷。但是，Northern分析的结果支持对nop-1基因表达的轻度和基于条件的调节，而毕赤酵母中异源表达的NOP-1蛋白通过使用全反式[3H]视网膜进行标记，表明NOP-1的功能是视紫红质在N.crassa光生物学中的作用。

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