A protein-protein interaction map of yeast RNA polymerase III

A F.PPRES; J.-F.BRIAND; O.GADAL

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A protein-protein interaction map of yeast RNA polymerase III

机译：酵母RNA聚合酶III的蛋白质相互作用图谱

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The structure of the yeast RNA polymerase (pol) III was investigated by exhaustive two-hybrid screening using a library of random genomic fragments fused to the Ga14 activation domain. This procedure allowed us to identify contacts between individual polypeptides, localize the contact domains, and deduce a protein-protein interaction map the multisubunit enzyme. In all but one case, pol III subunits were able to interact in vivo with one or sometimes two partner subunits of the enzyme or with subunits of TFIIIC. Four subunits that are common to pol I, II, and III(ABC27, ABC14.5, ABC10α, and ABC10β) two that are common to pol I and III (AC40 and AC19), and one pol III-specific subunit (CII) can associate with defined regions of the two large subunits. These regions overlapped with highly conserved domains. These regions overlapped with highly conserved domains. C53, an pol III-specific subunit. interacted with a 37-kDa polypeptide that copurifies with the enzyme and therefore appears to be a unique pol III subunit (C37). Together with parallel interaction studies based on dosage-dependent suppression of conditional mutants, our data suggest a model of the pol III preinitiation complex.

机译：酵母RNA聚合酶（pol）III的结构是通过使用与Ga14激活域融合的随机基因组片段文库进行详尽的两杂交筛选研究的。该程序使我们能够识别单个多肽之间的接触，定位接触域，并推导蛋白质-蛋白质相互作用图谱的多亚基酶。在除一种情况外的所有情况下，pol III亚基都能够在体内与该酶的一个或有时两个伙伴亚基或与TFIIIC的亚基相互作用。 pol I，II和III共有四个亚基（ABC27，ABC14.5，ABC10α和ABC10β），pol I和III共有两个亚基（AC40和AC19），以及一个pol III特异性亚基（CII）可以与两个大亚基的定义区域关联。这些区域与高度保守的域重叠。这些区域与高度保守的域重叠。 C53，pol III特有的亚基。与与该酶共纯化的37 kDa多肽相互作用，因此似乎是独特的pol III亚基（C37）。结合基于条件突变体剂量依赖性抑制的平行相互作用研究，我们的数据提出了pol III预起始复合物的模型。

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《Proceedings of the National Academy of Sciences of the United States of America》 |1999年第14期|7815-7820|共6页
作者
A F.PPRES; J.-F.BRIAND; O.GADAL;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
saccharomyces cerevisidetwo-hybrid system; biochemistry; biochemistry;

机译：酿酒酵母双杂交系统;生化;生化;

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1. Protein-protein interactions mapped by artificial proteases: where sigma factors bind to RNA polymerase [J] . Datwyler SA., Meares CF. Trends in biochemical sciences . 2000,第9期

机译：人工蛋白酶定位的蛋白质-蛋白质相互作用：σ因子与RNA聚合酶结合
2. Protein-protein interactions mapped by artificial proteases: where sigma factors bind to RNA polymerase (vol 25, pg 408, 2000) [J] . Datwyler., Meares. Trends in biochemical sciences . 2000,第10期

机译：人工蛋白酶定位的蛋白质-蛋白质相互作用：σ因子与RNA聚合酶结合（第25卷，第408页，2000年）
3. Genetic interactions within TFIIIC, the promoter-binding factor of yeast RNA polymerase III. [J] . Rozenfeld S, Thuriaux P Molecular biology reports . 2001,第4期

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6. A protein–protein interaction map of yeast RNA polymerase III [O] . A. Flores, J.-F. Briand, O. Gadal, 1999

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8. Novel Protein-Protein Interactions of the Yersinia pestis Type III secretion System Elucidated With a Matrix Analysis by Surface Plasmon Resonance and Mass Spectrometry [R] . Swietnicki, W. , O'Brien, S. , Holman, K. , 2004

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A protein-protein interaction map of yeast RNA polymerase III

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