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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Abscisic acid induces oscillations in guard-cell cytosolic free calcium that trivolve phosphoinositide-specific phospholipase C
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Abscisic acid induces oscillations in guard-cell cytosolic free calcium that trivolve phosphoinositide-specific phospholipase C

机译：脱落酸诱导保卫细胞胞质游离钙的振荡，从而使磷酸肌醇特异性磷脂酶C进化

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Oscillations in cytosolic free Ca~(2+) concentra- tion ([Ca~(2+)]_(cyt)) are an important component of Ca~(2-)-based signal transduction pathways. This fact has led us to investigate whether oscillations in [Ca~(2+)]_(cyt) are involved in the response of stomatal guard cells to the plant hormone abscisic acid (ABA). We show that ABA induces oscillations in guard-cell [Ca~(2+)]_(cyt) The pattern of the oscillations depended on the ABA concentra- tion and correlated with the final stomatal aperture. We exam- ined the mechanism by which ABA generates oscillations in guard-cell [Ca~(2+)]_(cyt) by using 1-(6-{[17#beta#-3-methoxyestra- 1,3,5 (1O) -trien- 17-yl] amino } hexyl) -1H-pyrrole-2,5-dione (U- 73122), an inhibitor of phosphoinositide-specific phospholipase C (PI-PLC)-dependent processes in animals. U-73122 inhibited the hydrolysis of phosphatidylinositol 4,5-bisphosphate by a recombinant PI-PLC, isolated from a guard-cell-enriched cDNA library, in a dose-dependent manner. This result confirms that U-73122 is an inhibitor of plant PI-PLC activity U-73122 inhib- ited both ABA-induced oscillations in [Ca~(2+)]_(cyt) and stomatal closure. In coutrast, U-73122 did not inhibit external Ca~(2+)- induced oscillations in guard-cell [Ca~(2+)] _(cyt) and stomatal closure. Furthermore, there was no effect of the inactive analogue 1-(6- {[17#beta#-3-methoxyestra-1,3,5 (10) -trien-17-yl] amino}hexyl) -2,5- pyrrolidinedione on recombinant PI-PLC activity or ABA- induced and external Ca~(2+)-induced oscillations in [Ca~(2+)]_(cyt) and stomatal closure. This lack of effect suggests that t
机译：胞浆中游离Ca〜（2+）浓度（[Ca〜（2 +）] _（cyt））的振荡是基于Ca〜（2-）的信号转导途径的重要组成部分。这一事实使我们研究了[Ca〜（2 +）] _（cyt）的振荡是否参与了气孔保卫细胞对植物激素脱落酸（ABA）的反应。我们证明了ABA诱导保护细胞[Ca〜（2 +）] _（cyt）的振荡。振荡的模式取决于ABA的浓度，并与最终的气孔孔径相关。我们使用1-（6-{[17＃beta＃-3-methoxyestra-1,3,5]检查了ABA在保卫细胞[Ca〜（2 +）] _（cyt）中产生振荡的机制。 （1O）-三烯-17-基]氨基}己基）-1H-吡咯-2,5-二酮（U- 73122），一种磷酸肌醇特异性磷脂酶C（PI-PLC）依赖性动物的抑制剂。 U-73122通过重组PI-PLC抑制了磷脂酰肌醇4,5-二磷酸的水解，该重组PI-PLC以剂量依赖的方式从富含保卫细胞的cDNA文库中分离出来。该结果证实，U-73122是植物PI-PLC活性的抑制剂，U-73122抑制了ABA诱导的[Ca〜（2 +）] _（cyt）振荡和气孔关闭。与此相反，U-73122不能抑制外部Ca〜（2+）诱导的保卫细胞[Ca〜（2 +）] _（cyt）振荡和气孔关闭。此外，无活性类似物1-（6-{[[[17＃beta＃-3-甲氧基estra-1,3,5（10）-三烯-17-基]氨基}己基）-2,5-没有影响。吡咯烷二酮对重组PI-PLC活性或ABA诱导的和外部Ca〜（2+）诱导的[Ca〜（2 +）] _（cyt）振荡和气孔关闭。这种缺乏效果表明

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