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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >A Holliday junction resolvase from Pyrococcus furiosus: Functional similarity to Escherichia coli RuvC provides evidenbe for conserved mechanism of homologous recombination in Bacteria, Eukarya, and Archaea
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A Holliday junction resolvase from Pyrococcus furiosus: Functional similarity to Escherichia coli RuvC provides evidenbe for conserved mechanism of homologous recombination in Bacteria, Eukarya, and Archaea

机译：激烈热球菌的霍利迪连接酶解酶：与大肠杆菌RuvC的功能相似性为细菌，真核生物和古细菌的同源重组的保守机制提供了证据

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摘要

The Holliday junction is an essential intermediate of homologous recombination. RecA of Bacteria, Rad51 of Eukarya, and RadA of Archaea are structural and funnctional homologs. These proteins play a pivotal role in the formation or Holliday junctions from two homologous DNA duplexes. RuvC is a specific endonuclease that resolves Holliday junctions in Bac- teria. A Holliday junction-resolving activity has been found in both yeast and mammalian cells. To examine whether the paradigm of homologous recombination apply to Archaea, we assayed and found the activity to resolve a synthetic Holliday Junction in crude extract of pyrococcus furiosus cells. The gene, the (Holliday junction cleavage), encodes a protein composed of 123 amino acids, whose sequence is not similar to that of any proteins with known function. However, all four archaea, whose total genome sequences have been published, have the homologous genes. The purified Hjc protein cleaved the recombination intermediates formed by RecA in vitro. These results support the notion that the formation and resolution of Holliday Junction is the common mechanism of homologous recombination in the three domains of life.
机译：霍利迪结是同源重组的重要中间体。细菌的RecA，Eukarya的Rad51和古细菌的RadA是结构和功能上的同源物。这些蛋白质在两个同源DNA双链体的形成或霍利迪连接中起关键作用。 RuvC是一种特异的核酸内切酶，可分解细菌中的霍利迪连接。在酵母和哺乳动物细胞中均发现了霍利迪结的分解活性。为了检查同源重组的范式是否适用于古生菌，我们进行了分析，并发现了解决其在激烈热球菌细胞粗提物中合成霍利迪交汇点的活性。该基因（霍利迪结切割）编码一种由123个氨基酸组成的蛋白质，其序列与任何具有已知功能的蛋白质的序列均不同。但是，所有四个已公开其全部基因组序列的古细菌都具有同源基因。纯化的Hjc蛋白在体外切割了由RecA形成的重组中间体。这些结果支持了霍利迪结的形成和分解是生命三个域中同源重组的常见机制的观点。

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