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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >The Arabidopsis SKP1-LIKE1 gene is essential for male meiosis and may control homologue separation
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The Arabidopsis SKP1-LIKE1 gene is essential for male meiosis and may control homologue separation

机译：拟南芥SKP1-LIKE1基因对于男性减数分裂至关重要，并可能控制同源物分离

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摘要

The yeast and human SKP1 genes regulate the mitotic cell cycle but are not yet known to be required for meiosis. Nine ArabiVopsis SKP1 homologues have been uncov- ered and are named ASK1 through ASK9. Here, we report the isolation and characterization of a male sterile Arabiyopsis mutant and show that the mutant defect was caused by a Ds transposon insertion into the ASK1 gene. In the askl-l mutant, abnormal microspores exhibit a range of sizes. Furthermore, during mutant male meiosis, although homologous chromo- some pairing appeared normal at metaphase I, chromosome segregation at anaphase I is unequal, and some chromosomes are abnormally extended. Therefore, in askl-1, at least some homologues remain associated after metaphase I. In addition, immunofluorescence microscopy indicates that the mutant spindle morphology at both metaphase I and early anaphase I is normal, thus, the abnormal chromosome segregation is not likely caused by a spindle defect. Because the yeast Skplp is required for targeting specific proteins for ubiquitin- mediated proteolysis, we propose that ASK1 controls homo- logue separation by degrading or otherwise removing a pro- tein that is required directly or indirectly for homologue association before anaphase I.
机译：酵母和人类SKP1基因调节有丝分裂细胞周期，但尚不知道减数分裂是必需的。尚未发现9个ArabiVopsis SKP1同源物，命名为ASK1至ASK9。在这里，我们报告了雄性不育拟南芥突变体的分离和表征，并表明该突变体缺陷是由Ds转座子插入ASK1基因引起的。在ask1-1突变体中，异常的小孢子表现出一定范围的大小。此外，在突变的雄性减数分裂过程中，尽管同源染色体在中期I出现了一些配对正常，但在后期I的染色体分离却不相等，并且某些染色体异常地延伸。因此，在askl-1中，在中期I之后至少有一些同源物保持关联。此外，免疫荧光显微镜检查表明，中期I和后期I早期的突变纺锤体形态都是正常的，因此，异常染色体分离很可能不是由主轴缺陷。由于酵母Skplp是靶向泛素介导的蛋白水解的特定蛋白质所必需的，因此我们建议ASK1通过降解或以其他方式除去在阶段I之前直接或间接进行同源物缔合的蛋白质来控制同源物分离。

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