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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS)
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Identification of Mycobacterium tuberculosis RNAs synthesized in response to phagocytosis by human macrophages by selective capture of transcribed sequences (SCOTS)

机译：通过选择性捕获转录序列（SCOTS），鉴定人巨噬细胞对吞噬作用反应而合成的结核分枝杆菌RNA

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摘要

A widely applicable, positive cDNA selection method was developed to identify RNAs synthesized by Myco- bacterium tuberculosis in response to phagocytosis by cultured human primary macrophages. cDNAs for sigE and sigH (al- ternative sigma factors), aceA (isocitrate lyase), ponA (class I Penicillin-binding protein), pks2 (polyketide synthase), uvrA (UvrABC endonuclease), and ctpV (putative cation trans- porter) were obtained from macrophage-grown bacteria. cD- NAs for ORFs Rv3070, Rv3483c, Rv0903c (encoding a putative bacterial two-component transcriptional activator), and Rv0170 of the mcel virulence operon also were obtained from phagocytized bacilli. cDNAs for these genomic regions were not obtained from approximately 1,000-fold more bacteria grown in laboratory broth. Methods described here, which have identified M.tuberculosis genes expressed in response to host interaction, will allow the study of gene expression in a variety of microorganisms, including expression resulting from interaction with human tissues in natural disease states.
机译：开发了一种广泛适用的阳性cDNA选择方法，以鉴定结核分枝杆菌合成的RNA，以响应培养的人类原代巨噬细胞的吞噬作用。 sigE和sigH（替代sigma因子），aceA（异柠檬酸裂合酶），ponA（I类青霉素结合蛋白），pks2（聚酮化合物合酶），uvrA（UvrABC核酸内切酶）和ctpV（假定阳离子转运蛋白）的cDNA。从巨噬细胞生长的细菌获得。还从吞噬的杆菌获得了ORF Rv3070，Rv3483c，Rv0903c（编码假定的细菌两成分转录激活因子）和mcel毒力操纵子的Rv0170的cD-NA。这些基因组区域的cDNA不能从实验室培养液中生长的大约1,000倍的细菌中获得。此处描述的方法已经鉴定出响应宿主相互作用而表达的结核分枝杆菌基因，将允许研究多种微生物中的基因表达，包括在自然疾病状态下与人体组织相互作用产生的表达。

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