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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Differential colocalization of estrogen receptor β (ERβ) with oxytocin and vasopressin in the paraventricular and supraoptic nuclei of the female rat brain: An immunocytochemical study
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Differential colocalization of estrogen receptor β (ERβ) with oxytocin and vasopressin in the paraventricular and supraoptic nuclei of the female rat brain: An immunocytochemical study

机译:雌性大鼠脑室旁和视上核中雌激素受体β(ERβ)与催产素和加压素的共定位差异:免疫细胞化学研究

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摘要

Evidence exists for the localization of the newly identified estrogen receptor β (ERβ) within the rat paraventricular nucleus (PVN) and supraoptic nucleus (SON), regions which lack ER. Presently, we investigate whether ERβ-like-immunoreactivity (-ir) is found within cells of several major neuropeptide systems of these regions. Young adult Sprague-Dawley rats were ovariectomized (OVX), and 1 week later half of the animals received estradiol-17β (E). Dual-label immunocytochemistry was performed on adjacent sections by using an ERβ antibody, followed by an antibody to either oxytocin (OT), arginine-vasopressin (AVP), or cortico- tropin releasing hormone.
机译:有证据表明,新发现的雌激素受体β(ERβ)在缺乏ER的大鼠心室旁核(PVN)和视上核(SON)内定位。目前,我们调查是否在这些区域的几个主要神经肽系统的细胞内发现了ERβ样免疫反应性(-ir)。将年轻的成年Sprague-Dawley大鼠切除卵巢(OVX),1周后一半的动物接受雌二醇17β(E)。通过使用ERβ抗体,然后是抗催产素(OT),精氨酸加压素(AVP)或促肾上腺皮质激素释放激素的抗体,对相邻切片进行双标记免疫细胞化学分析。

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