Expression of the multiple interferon-τ (IFN-τ) genes is restricted to embryonic trophectoderm of ruminant ungulate species for a few days in early pregnancy. The promoter regions of these genes are highly conserved. A proximal (bp -91 to -69) sequence has been implicated in controlling trophoblast-specific expression. Here it was used as a target for yeast one-hybrid screening of a day 13 con- ceptus cDNA library. Two transcription factors of the Ets family, Ets-2 and GABPτ, were identified, consistent with the observation that active ovine IFN-τ genes contain a single 10-bp Ets motif (core: GGAA) in the proximal segment, whereas three known inactive ovine genes contain a mutated core motif (TGAA).
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机译：妊娠早期几天内，多种干扰素-τ（IFN-τ）基因的表达仅限于反刍有蹄类物种的胚滋养外胚层。这些基因的启动子区域是高度保守的。近端（bp -91至-69）序列与控制滋养层特异性表达有关。在这里，它被用作第13天概念cDNA文库的酵母单杂交筛选的靶标。确定了Ets家族的两个转录因子Ets-2和GABPτ，这与观察到的活性绵羊IFN-τ基因在近端片段中包含单个10 bp Ets模体（核心：GGAA）相一致，而三个已知的非活性绵羊基因包含一个突变的核心基序（TGAA）。
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