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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >DIRECT IN VIVO MEASUREMENT OF TARGETED BINDING IN A HUMAN TUMOR XENOGRAFT
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DIRECT IN VIVO MEASUREMENT OF TARGETED BINDING IN A HUMAN TUMOR XENOGRAFT

机译：人体异种移植中目标结合的体内直接测量

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摘要

Binding is crucial to the function of most biologically active molecules, but difficult to quantify directly in living tissue, To this end, fluorescence recovery after photobleaching was used to detect the immobilization of fluorescently labeled ligand caused by binding to receptors in vivo, Measurements of mAb affinity to target antigen within human tumor xenografts revealed a saturable binding isotherm, from which an in vivo carcinoembryonic antigen density of 0.56 nmol/g (5.0 x 10(5)/cell) and an association constant of K-a less than or equal to 4 x 10(7) M(-1) were estimated. The present method can be adapted for in vivo studies of cell signaling, targeted drugs, gene therapy, and other processes involving receptor-ligand binding. [References: 35]
机译：结合对于大多数生物活性分子的功能至关重要，但很难直接在活组织中定量。为此，光漂白后的荧光恢复被用于检测由体内结合受体引起的荧光标记配体的固定化，mAb的测量对人肿瘤异种移植物中靶抗原的亲和力显示出可饱和的结合等温线，从中可得体内癌胚抗原密度为0.56 nmol / g（5.0 x 10（5）/细胞），且缔合常数Ka小于或等于4 x估计10（7）M（-1）。本方法可以适用于细胞信号转导，靶向药物，基因治疗以及涉及受体-配体结合的其他过程的体内研究。 [参考：35]

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