Development of 'substrate-trapping' mutants to identify physiological substrates of protein tyrosine phosphatases

Andrew J. Flint; Tony Tiganis; David Barford

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Development of 'substrate-trapping' mutants to identify physiological substrates of protein tyrosine phosphatases

机译：“底物捕获”突变体的开发，以鉴定蛋白质酪氨酸磷酸酶的生理底物

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The identification of substrates of protein ty- rosine phosphatases (PTPs) is an essential step toward a com- plete understanding of the physiological function of members of this enzyme family. PTPs are defined by a conserved catalytic domain harboring 27 invariant residues. From a mutagenesis study of these invariant residues that was guided by our knowl- edge of the crystal structure of PTP1B, we have discovered a mutation of the invariant catalytic acid (Asp-181 in PTP1B) that converts an extremely active enzyme into a "substrate trap."

机译：蛋白质酪氨酸磷酸酶（PTP）底物的鉴定是全面了解该酶家族成员生理功能的重要步骤。 PTP由保守的催化结构域定义，该结构域包含27个不变残基。根据我们对PTP1B晶体结构知识的了解，对这些恒定残基进行了诱变研究，我们发现了恒定催化酸（PTP1B中的Asp-181）发生了突变，该突变将一种极为活跃的酶转化为“底物”。陷阱。”

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《Proceedings of the National Academy of Sciences of the United States of America》 |1997年第5期|p.1680-1685|共6页
作者
Andrew J. Flint; Tony Tiganis; David Barford;
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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
中图分类自然科学总论;
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1. DEVELOPMENT OF SUBSTRATE-TRAPPING MUTANTS TO IDENTIFY PHYSIOLOGICAL SUBSTRATES OF PROTEIN TYROSINE PHOSPHATASES [J] . Flint AJ., Barford D., Tonks NK., Proceedings of the National Academy of Sciences of the United States of America . 1997,第5期

机译：开发用于蛋白质酪氨酸磷酸酶生理基质的底物诱集剂
2. Yeast substrate-trapping system for isolating substrates of protein tyrosine phosphatases: Isolation of substrates for protein tyrosine phosphatase receptor type z. [J] . Fukada M, Kawachi H, Fujikawa A, Methods: A Companion to Methods in Enzymology . 2005,第1期

机译：用于分离蛋白质酪氨酸磷酸酶底物的酵母底物捕获系统：分离蛋白质酪氨酸磷酸酶受体z型的底物。
3. A New Highly Efficient Substrate-trapping Mutant of Protein Tyrosine Phosphatase 1B (PTP1B) Reveals Full Autoactivation of the Insulin Receptor Precursor [J] . Samira Boubekeur, Nicolas Boute, Patrick Pagesy, The Journal of biological chemistry . 2011,第22期

机译：蛋白质酪氨酸磷酸酶1B（PTP1B）的新的高效底物捕获突变体揭示了胰岛素受体前体的全部自动激活
4. Actinodaphnine and Rutacridone as New T-Cell Protein Tyrosine Phosphatase Inhibitors for Drug Development of Obesity [C] . Y Fitrianingrum, D Indarto, R Kusumawati Annual Basic Science International Conference . 2019

机译：Actinodaphnine和Rutacridone作为新的T细胞蛋白酪氨酸磷酸酶抑制剂，用于肥胖的药物发育
5. Substrate Specificity of Protein Tyrosine Phosphatase-like myo-Inositol Phosphatases: PhyA in Complex with Ins(1,2,4,5,6)P5, Ins(1,3,4,5)P4, and Ins(1,4,5)P3 [D] . Bruder, Lisza M. 2019

机译：蛋白质酪氨酸磷酸酶样肌醇磷酸酶的底物特异性：与Ins（1,2,4,5,6）P5，Ins（1,3,4,5）P4和Ins（1,4， 5）P3
6. Development of substrate-trapping mutants to identify physiological substrates of protein tyrosine phosphatases [O] . Andrew J. Flint, Tony Tiganis, David Barford, 1997

机译：开发底物诱捕突变体以鉴定蛋白质酪氨酸ros磷酸酶的生理底物
7. Development of "substrate-trapping" mutants to identify physiological substrates of protein tyrosine phosphatases [O] . A. J. Flint, T. Tiganis, D. Barford, 1997

机译：发展“底物捕获”突变体以鉴定蛋白质酪氨酸磷酸酶的生理基质

Development of 'substrate-trapping' mutants to identify physiological substrates of protein tyrosine phosphatases

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