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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >IDENTIFICATION OF AN ALTERNATIVE FORM OF HUMAN LACTOFERRIN MRNA THAT IS EXPRESSED DIFFERENTIALLY IN NORMAL TISSUES AND TUMOR-DERIVED CELL LINES
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IDENTIFICATION OF AN ALTERNATIVE FORM OF HUMAN LACTOFERRIN MRNA THAT IS EXPRESSED DIFFERENTIALLY IN NORMAL TISSUES AND TUMOR-DERIVED CELL LINES

机译：鉴定在正常组织和肿瘤细胞系中差异表达的人乳铁蛋白MRNA的替代形式

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Lactoferrin (LF), traditionally known as an iron-binding protein present in high concentrations in milk and various secretions, has emerged as a multifunctional protein involved in many aspects of the host defense against infection, Recently, LF has been shown to inhibit the growth of solid tumors and reduce experimental metastasis in mice, suggesting that LF also mag play a role in the defense against tumorigenesis. Here we provide the sequence of the cDNA and promoter region, the chromosome assignment, and tissue expression pattern of a novel form of LF mRNA (Delta LF), The sequence of Delta LF mRNA is nearly identical to that of LF mRNA; however, at the 5' end, me find a novel sequence that replaces the N-terminal signal peptide sequence of LF mRNA, We map the Delta LF mRNA to human chromosome 3 and find that both Delta LF and LF sequences colocalize to the same cloned 90- to 150-kb genomic DNA fragment. We further show that the Delta LF mRNA is the product of alternative splicing of the LF gene and likely is specified by use of an alternative promoter, Although me find Delta LF mRNA at various levels in 20 of 20 adult and fetal human tissues, me do not find Delta LF mRNA in any of 14 diverse tumor-derived cell lines. [References: 53]
机译：乳铁蛋白（LF），通常被称为铁结合蛋白，存在于牛奶和各种分泌物中，浓度高，已成为一种多功能蛋白，参与宿主抵抗感染的许多方面。最近，LF被证明可抑制生长实体瘤的发生并减少了小鼠的实验转移，这表明LF在抗肿瘤发生的防御中也起着重要作用。在这里，我们提供了一种新型的LF mRNA（Delta LF）的cDNA和启动子区域的序列，染色体分配和组织表达模式。DeltaLF mRNA的序列与LF mRNA的序列几乎相同。然而，在5'端，我发现了一个新的序列，该序列取代了LF mRNA的N端信号肽序列，我们将Delta LF mRNA映射到人类3号染色体，发现Delta LF和LF序列共定位于同一克隆90至150 kb的基因组DNA片段。我们进一步表明，Delta LF mRNA是LF基因的可变剪接产物，可能通过使用替代启动子来指定。尽管我发现20个成人和胎儿人类组织中有20个处于不同水平，但Delta LF mRNA确实存在在14种不同的肿瘤来源的细胞系中均未发现Delta LF mRNA。 [参考：53]

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